Study On Extraction Of Ceramide Homolog From Konjac

Konjac is a kind of raw plant material, which is used as a drug ingredient as well as a food to eat. Ceramides have functions such as moisturizing, whiting, anti-aging, anti-tumor and antivirus. They are widely applied in food, medicine and makeup industries. With the konjac as the raw material, this article mainly talks about the extraction conditions, separation and purification methods and functional experiments of ceramides from konjac. At the same time, a preparatory analysis and identification of the konjac ceramides was done by IR, UV, TLC and HPLC. The results are as follows:1. Use Soxhet extraction to research the extraction conditions. The effects of single factors to extraction rate of ceramides, such as extraction temperature, ratio of liquid to material, the concentration of solvent ethanol and time were investigated: The best technical conditions for ceramides extraction were obtained through orthogonal experiments as follows:temperature 92℃,95% ethanol, time 12 h and liquid material ratio 1:5. In this condition, extraction rate of ceramides was 1.46%.2. Researching separation and purification methods of ceramides by Silica Column Chromatography. The results of the experiments showed that:separation and purification of ceramides were effected by the flow rate and the polarity of the eluant and the amount of the sample. The flow rate of the eluant was 0.8 mL/min, the amount of the sample was 0.7 g, the eluant was V(ethyl acetate):V(petroleum)=7:3. Under these conditions, the separation effect was good.3. Preparatory analysis and identification of the konjac ceramides was done by IR, UV, TLC and HPLC. The infrared mapping spectrometer carried the characteristic peak of the function groups of the ceramides, which demonstrates that the purified sample contains ceramides; the TLC certifies that the products are ceramides; the standard sample and product of ceramides are analyzed by HPLC. Through the compare the HPLC graphs of standard sample and product, we found that the ceramides were contained in the purified sample, with a small amounts of impurities.4. Through the anti-oxidation experiment of the fat and oil, we found that the konjac ceramides had clearly anti-oxidation impact on animal fat and vegetable oil; The ceramides have a favorable moisturizing property, which is superior than the common glycerin and olive oil.