Studies On Identification Of A Wetland Plant Rhizosphere Microorganism LF2, Optimization Of The Fermental Conditions And Application Effect In Constructed Wetland

Constructed wetlands are a kind of low-cost and natural alternative to technical methods of wastewater treatment. They are becoming a very useful technology for the treatment of all kinds of wastewaters. An important part of the treatment in constructed wetlands is attributable to the existence and activity of plants and microorganisms. Recent studies showed that the main role in the transformation and mineralization of nutrients and organic pollutants is played not by plants, but by microorganisms. The microbiology is the most important factor in treatment wetlands,and influencing the removal of pollutants in these systems.This article use the rhizosphere microorganisms which have a good effect to degradation of sanitary wastewater COD, making16S rDNA sequencing and morphology, identified the strain as Lysinibacillus fusiformis. Put the bacteria into the small-scale wetland Outside the door, when adding the volume ratio of sewage and bacteria is 1:10000, the experimental group which adding bacteria, the COD removal rate was 94.8%. The control group without put bacteria the COD highest rate of 73.1%. Analysis of variance showed that the removal rate of COD in the experimental group with bacteria was significantly higher than the control group without bacteria (P<0.05), and an average increase of more than 20%, indicating that the bacteria can significantly improve removal rate of COD in construction wetlands wastewater, with obvious practical value.Rhizosphere bacteria L. fusiformis not only have a higher COD degradation, but also bacteria of the genus Bacillus. Bacillus bacteria form dormant spores, spores on the environment was well tolerated, strong resistance, non-pathogenic, and can withstand filtration, drying and other post-processing techniques, and be able to save time for dealing promote the use of large-scale domestic wastewater. In the fifth part of the single factor experiment L. fusiformis in the fermentation medium was studied, designed to greatly improve the production level of the bacterial spores, in order to lay the foundation to reduce production costs. The optimum medium composition was as follows:the concentration of glucose 12g/L, yeast extract concentration of 10.5 g/L, KH2PO4 concentration of 3.0 g/L and the concentration of MnSO4·H2O 0.38 g/L.