The Extraction And Purification Of Chinese Gallotannins And Its Antibiosis And Antimutigenicity Effect

Galla chinensis is the gall produced by some parasitic aphids on the Rhus leaves, P.potaninii leaves or R.punjabensis leaves of the family Anacardiaceae. Galla chinens is a traditional Chinese herb. Its'main chemical compositions are gallotannins, fatty oil, resin, protein, fat, starch, waxiness, and mineral substance, etc. Chinese gallotannins(CGTs) are the main bioactive and the most compounds, which have a research area including extraction, purification, antioxidant, bacteriostat, component analysis, and structuralidentification. few research are related to the antimutigenicity effect of CGTs, and in respects of extraction, studies have focused on the selection of different solvent.In this paper, CGTs was taken to be the research object. Orthogonal array design was employed to optimize ultrasonic extraction condition. the effects of volume fraction of solvent, liquid-to-solild ratio, ultrasonic time, extractive temperature, ultrasonic power on the yield of CGTs was investigated, the purification process of CGTs was also studied. The effects of CGTs on mutagenicity and antimutagenicity were studied by Ames test; the antibacterial activity of CGTs was studied by oxford cup method compared with TP and penicillin.their minimum inhibitory concentration(MIC) against seven common food spoilage microbes was established by double broth dilution method. what is more, the effects of temperature and pH on the antibacterial activity of CGTs was observed and researched.Principal research achievements are as follows:(1) Orthogonal array design was employed to optimize the ultrasonic extraction of Chinese Gallotannins(CGTs),and the purification process of CGTs was also studied in this paper. The optimal extraction condition of CGTs are as follows:ultrasonic power is 200 W,60% acetone as extractant, liquid-to-solid ratio is 24:1, extraction time is 30 min, temperature is 40℃; The CGTs can obtain maximum extraction efficiency as 92.27% in two times extraction.The purification degree of CGTs was up to 92.89% with organic solvents. (2) The effects of CGTs on mutagenicity and antimutagenicity were studied by Ames test. The results showed that no mutagenicity was observed with CGTs. Comparing with positive groups, CGTs groups could decrease the colonies in TA97,TA98,TA100 and TA102 strains. Moreover, the antimutigenicity was correlated to the applied dosage(200～3500μg/dish) in Ames test. we demonstrated that CGTs possessed significant antimutagenic properties.(3) the antibacterial activity of Chinese Gallotannins(CGTs) was studied by oxford cup method.the results showed that CGTs had prominent antimicrobial activities on Bacillus pyocyaneus, Bacullus typhi murium, Lister mocytogenes, Candida albicans, Penicilliumexpansum, Alicyclobacillus acidoterrestris, and Bacillus megaterium. Their MIC values were 5.0000g/L, 2.50g/L,5.00g/L,2.50g/L,5.00g/L,5.00g/L and 2.50g/L. to Bacillus megaterium, inhibition effect is:penicillin>CGTs=TP, to the other bacteria, inhibition effect is:penicillin> CGTs>TP. CGTs can attain perfect bacteriostatic efficacy when the temperature is below 45℃,but when the temperature is higher than 50℃,CGTs completely loose antibacterial activity. CGTs can inhibit the bacteria completely when the pH was 3-5, so acidiccondition is more beneficial to the antibacterial activity of CGTs.