The Study Of Chemiluminescent Immunoassay Based On TCPO-H₂O₂-PHPPA Dimer Chemiluminescent System

This thesis consists of two parts:Chapter one:The reviewIn this part, a review about fluoroimmunoassay(FIA), Chemiluminescence immunoassay(CLIA) marked with fluorescent substance, Chemiluminescence immunoassay(CLIA) based on fluorescent derivatization reaction, the application and development trends of these methods were summarized.Chemical luminescence immunoassay(CLIA), combined chemiluminescence method with immunoassay method, based on the theory of radioimmunoassay,is a non-radiolabeled immunoassay by marking the agent as a tracer signal, used to detect antibodies, antigens, haptens, enzymes, fatty acid, hormone, vitamin and so on. Due to the main advantages of high sensitivity, wide dynamic range, non-radiation, simple instrument equipment, convenient operation.rapid analysis and automatic control, CLIA has raised worldwide interest. Especially the novel CLIA marked with fluorescent substance and based on fluorescent derivatization reaction has raised worldwide interest using in immunoassay.Now the development trend of chemical luminescence immunoassay(CLIA) is to synthesize new luminous markers, study and explore chemiluminescence reaction with higher luminescence quantum yields and more sensitive.As one of most widely analytical methods, Chemical luminescence immunoassay has the advantages with the high sensitivity of chemiluminescence method and the high specificity of immunoassay method.Chapter two:Research reportsThe research part includes two sections. In the first section, A typical "sandwich type" immunoassay was used to detect staphylococcal enterotoxin B (SEB), bovine serum albumin (BSA) and carcinoembryonic antigen (CEA) based on the TCPO-H202-glyoxaline-PHPPA Dimer chemiluminescent System. Compared with conventional ELISA assay and the luminol chemiluminescence system, the oxalate chemiluminescent system is more sensitive and the chemiluminescence quantum yield is higher. A novel sensitive method based on ELISA using a pair of monoclonal antibodies (mAbs) that recognizes different epitopes for enhancing the specificity of immunoassay. In the second section, by "indirect competitive ELISA" immune response,Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a method for analysis of Clenbuterol and Quinolones based on the TCPO-H2O2-PHPPA Dimer chemiluminescent System.The result show that the oxalate chemiluminescent system is more sensitive and the chemiluminescence quantum yield is higher.It makes the immunoassay highly sensitive.