Study On Preparation Of Nao-Qing Nasal Spray Emulsion

The main component of Nao-Qing Nasal Spray Emulsion, which is used to treatment of AIS, is Moschus, Notoginseng, Ligustici, Rhizoma, Borneol, et al. The feature is multiplicity compositions and multiplicity constitution. The emulsion formulation and nasal cavity dosage are combined in the research of Nao-Qing Emulsion, to make the drugs and nasal mucosa contact closely and continuously, in order to improve the bioavailability, increase compliance treating patients, especially to utilize the feature of dosage form-nasal spray, to strengthen the target to pars encephalica. It is suitable for the ischemic cerebrovascular inadequacies in the acute phase of the emergency treatment. There is none report about emulsion binding Chinese crude drug nasal spray.The best extraction tehcnics of muscone, Rhizoma and Mentha haplocalyx were gained by single factor investigation and orthogonal experiment,when effective component contents were as detection index. The result of verification experiment indicated that it was efficient and reproducible.In this paper, inclusion compound was made by gripping borneol with hydroxypropyl-β-cyclodextrin(HP-β-CD), in order to reduce the volatilization of borneol in the preparation.Effects of oil, emulsifier and stabilizer were studied by single factor test, and based on these we found the best formula by orthogonal design when refining soybean oil, emulsifier and stabilizer were as consideration factors, Ke as investigation index. Effects of emulsification temperature, emulsification time, speed of high-speed mixer, nanomizer working Pressure and cycle times were also investigated by single factor test, and then the orthogonal design was adopted to obtain the optimized preparation procedure.Gas chromatography(GC)method was set up to determine the concentration of muscone and borneol.TLC method was used to identify Notoginseng, Ligustici, Rhizoma, Borneol and Mentha haplocalyx.The physiochemical properties of Nao-Qing Nasal Spray Emulsion were studied. Gaussian volume-Wt diameter, zeta-potential, viscosity and pH value is 256±22nm, -23.50mV, 1. 367(10^-3Pa﹒s), 6.39 respectively.The results of accelerating and long-term stability tests indicated that the physical and chemical stability of Nao-Qing Nasal Spray Emulsion were fine and incompliance with the requirement of preparation stability, but it should be tightly preserved form light and heat. There was no creaming after Nao-Qing Nasal Spray Emulsion was centrifugated at speed of 4000 rpm for 15 min, and freezing-heating experiment showed Nao-Qing Nasal Spray Emulsion had better aggregations stability. Meanwhile, preliminary evaluation of the safety of Nao-Qing Nasal Spray Emulsion proved that there was no clear toxicity on mice.Experiments show that Nao-Qing Nasal Spray Emulsion, which improves the drug loading of fat-soluble drugs and reduces irritation, is stable, non-toxic formulation, in line with the requirements of external emulsion.