Studies On Analytical Methods Of Melamin-related Substances And Some Antibiotics By HPLC

The analysis of melamine-related substances and antibiotics in the environmental waters and body fluids is of importance for human health. The aim of this paper is to establish the new reverse phase high performance liquid chromatographic(RP-HPLC)methods for the effective separation and sensitive determination of melamine, cyanuric acid and cyromazine in the environmental waters, and levofxaxin, cefazolin and cefminox in human urine. It includes third chapters as follows:Chapter 1: The basic principle of reversed-phase ion pair chromatography. The technolgies concerned with the seperation and determination of melamine-related substances and some antibiotics in recent years are introduced.Chapter 2: A new method for effective separation and determination of cyanuric acid, melamine and cyromazine in the environmental waters by reversed phase high performance liquid chromatography (RP-HPLC) with diode array detector is developed. A KROMASIL? C18 column (250×4.6 mm I.D., 5μm) was used for RP-HPLC using a gradient elution procedure. The proposed method has the advantages of high sensitivity and precision, and can permit the detection of cyanuric acid and cyromazine at levels as low as 0.07μg/mL and melamine at levels as low as 0.03μg/mL in environmental water samples.Chapter 3: Levofxaxin, cefazolin and cefminox can be administered perorally or intravenously, and are excreted mainly into the urine. They can be combined to enhance pharmacodynamics. A new method for simultanous determination of levofxaxin, cefazolin and cefminox by reversed phase high performance liquid chromatography with diode array detector in human urine is developed. The proposed method is effective and accurate, and the sensitivity and linearity can meet the request of analysis of the test drugs in human urine at clinically relevant concentrations.Chapter 4: Conclusion.