Study On Glucose Sensor Based On Multi - Stage Pore

Part 1. The design and application of glucose biosensor based on hierarchical silica spheres (HS) with macroporous and microporous structuresHierarchical silica spheres (HS) with macroporous and microporous structures were synthesized successfully by surfactant self-assembly method for binary-solvent system using sodium metasilicate nonahydrate as silica source and cationic surfactant cetyltrimethylammonium chloride as a template in water-ethanol binary solvent system. Then a novel glucose biosensor based on the hierarchical silica spheres was constructed and evaluated for the electrochemical performance and glucose determination. The results showed that the fabricated glucose bioaenser displayed an excellen. ekctrocatalytic performance fc; glucose detection as well as good stability and repeatability. The amperometric response of HS-GOD modified electrode was linearly proportional to the concentration of glucose concentration in the range of 0.1-3.25 mM with a good sensitivity of 1.81 mA·M-1·cm-2 and a low detection limit of 0.05 mM at signal-to-noise ratio of 3.Part 2. The design and application of glucose biosensor based on amino-functionalized silica particles with tunable hierarchical mesoporous pores (HPSNs-NH2)Amino-functionalized silica particles with tunable hierarchical mesoporous pores (HPSNS-NH2) were synthesized successfully by surfactant self-assembly method for multiple-solvent system using tetraethyl orthosilicate as silica source, cationic surfactant cetyltrimethylammonium bromide as structure-directing agent and 3-aminopropyltriethoxysilane as aminosilane coupling agent in water-ethanol-ethyl ether ternary-solvent system. Then a novel glucose biosensor based on the hierarchical porous silica particles was constructed and evaluated for the electrochemical performance and glucose determination. The results showed that the fabricated glucose biosensor displayed an excellent eiectrocatalyuc performance for glucose detection as well as good stability and repeatability. The amperometric response of HPSNs-NH2-God modified electrode was linearly proportional to the concentration of glucose concentration in the range of 0.1-2.60 mM with a good sensitivity of 3.95 mA·M-1·cm2 and a low detection limit of 0.05 mM at signal-to-noise ratio of 3.Part 3. The design and application of glucose biosensor based on Au nanoparticles loaded silica particles with tunable hierarchical mesoporous pores (HPSNs-Au)Au nanoparticles loaded silica particles with tunable hierarchical mesoporous pores (HPSNs-Au) were synthesized successfully by self-assembly electrostatic adsorption method. Then a novel glucose biosensor based on the hierarchical porous silica particles was constructed and evaluated for the electrochemical performance and glucose determination. The silica particles was more biocompatible and offered a favorable microenvironment for facilitating the drect electron transfer between GOD and electrode. And the gold nancparticles loaded with solid particles were beneficial to improve the transfer rate between electron donor and electron acceptor and enhanced the conductivity of the membrane. The results showed that the fabricated glucose biosensor displayed an excellent electrocatalytic performance for glucose detection as well as good stability and repeatability. The amperometric response of HPSNs-Au-GOD modified electrode was linearly proportional to the concentration of glucose concentration in the range of 0.05-1.35 mM with a high sensitivity of 19.65mA·M-1·cm-2 and a low detection limit of 0.01 mM mM at signal-to-noise ratio of 3.