| The screening and determination of drugs in body fluids plays an important role in life sciences and pharmaceutical research. Currently, high performance liquid chromatography (HPLC) has been considered as a fast analytical technique for drug analysis of biological fluids. However, the biological fluids are very complicated, which contains much protein and other interference components. When the proteineous matrices are directly inject into the reversed stationary phases such as C18, protein denaturation would be occurs with an irreversible adsorption on the particles of the stationary phase, which causes a rapid deterioration of chromatographic performances, clogging of the column and interrupt the determination of the analytes. Moreover, due to the low concentrations of the analytes in biological samples, pretreatment and enrichment is needed, so the direct injection of the biological samples is not compatible with most chromatographic systems. The weak ion-exchange monolithic column developed in this work is desired to overcome the above problems.In this paper, an ion exchange monolithic column was prepared using methylacrylic acid (MAA) as a monomer and ethylene dimethacrylate (EDMA) as a cross linking agent, which could remove matrix compounds in biological fluid repeatedly, and at the same time the drugs was enrichment completed. As a result, the on-line clean-up and enrichment could be carry out when using this column as solid-phase extraction material and a C18 column as analytical column.In this paper, a weak ion exchange column was prepared with glycidyl methacrylate (GMA) as the monomer, and the epoxide groups of the monolithic column were modified by ethylene diamine, and then modified using chloroacetic acid. The obtained cation-exchange column was used as the solid-phase extraction material to determinate melamine in milk. This method saved production costs and time, is an economic, effective and rapid detection tool for melamine determination.
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