Research For The Influence Of Electromagnetic Fields To The Ice Formation Of Physiological Salt Solution

Ice formation inside or outside cells has been proposed to be a factor causing cryoinjury to cells or tissues during cryopreservation. How to control, reduce or eliminate the ice formation has been an important research topic in fundamental cryobiology.Common methods used to achieve vitrification involve the use of either an ultrarapid cooling rate or a very high concentration of cryoprotectants. Unfortunately, an ultrarapid cooling rate is very difficult to attain, introduction of high concentration of cryoprotectants can directly damage cells and tissues. For these reasons it is desirable to develop novel technologies to vitrify biomaterials using achievable cooling rates.In this paper the effect of alternating electric field and magnetic field on ice formation during the cooling process. The samples used in this study consisted of aqueous solutions of KMnO4. A set of magnetic system was design to produce an alternating magnetic field and a set of electrode system was design to produce an alternating electric field for sample. A simple microscope was modified for low-temperature observation by combining a semiconductor-refrigerating cavity. The relationship between the ice formation and these parameters of electric field frequency range, magnetic field frequency range and the rate of cooling was observed. Some optimized electromagnetic parameters will be explored for the vitrification of the solution. A qualitative analysis has been made by the mechanism of dielectric polarization and loss.