Preparation Of Microbial Fuel Cell Based On Wastewater Treatment And Its Fundamental Researches

Microbial fuel cell is a construct of use bacteria to generate electricity form the oxidation oforganic matter. Now the reacher still on the laboratory stage. Research mainly concentrated inhow to improve microbial fuel cell electrogenesis capacity and its influencing factors, in order toproviding scientific basis for microbial fuel cells practical application .This article focuses on the following three aspects of microbial fuel cells:Frist:Electricigens as the key of microbial fuel cell, we investigate the electricigen. Fristchose of appropriate strains which have higher electrogenesis capacity , then select the optimalgrow condition of dominant strain , such as substrate, temperature, pH, etc. Results showed thatusing wastewater treatment plants water as the source of strain, 3mol/l glucose as substrate,temperature of 30℃, pH value for 5.5 , were the optimal grown condition for the electricigen.Added FeCl3 to medium, 1mol/L FeCl3 select electricigen had higher electrogenesis capacity .Second:In order to improve the performance of microbial fuel cells and reduce the cost ofmicrobial fuel cells, we investigated the whole structure, the performance of single chambermicrobial fuel and double chambles microbial fuel cell, then studies the anode electrodematerials carbon cloth and porous grapHite plate, we also studied the membrane between anodeand cathode, such as proton exchange membrane, cationic exchange membrane, anion exchangemembrane on how they effect microbial fuel cells performance. Results showed that using singlechamber structure, using porous grapHite plate as the anode material, cation exchange membraneas the separator, can improved microbial fuel cell performance while reduce the cost .Third: Anode potential determined the growth and metabolism of electricigen, so in thispaper, we studied how the anode poised potential effect of the electricigen type, the effct of it onthe whole microbial fuel cell perfomancer and the effect on start up time, use scanning electronicmicroscope( SEM) to observe bacteria colony morpHosi, use cyclic voltam metry (CV) to studythe ellectricigen electrochemical activity, use polymerase chain reaction denaturing gradient gelelectropHoresis (PCR-DGGE). we studied the electricigen colony component . Results showedthat different anode poised potential can selected different electricigen which was proved by SEM, polar curve showed - 100mV as the optimization select anode poised potential, it canshorten the microbial fuel cell start–up time and we can get the highest power density, CVresults showed selected electricigen can direct electron transfer and had different matebolismpath. DGGE results showed that in the anode poised potential as -200mV it can only select onetype electricigen, this result accorded with our suppose if setting appropriate voltages can beselected pure strains .