Detection Of Resistance To Botrytis Cinerea And Its Isolation Of Biocontrol

As a worldwide disease, Botrytis cinerea poses a serious threat to the growth of vegetablecrops. Currently, the main controlling method of the Botrytis cinerea is chemical control, andbiological control is served as a supplement. In the mean time, agricultural control also plays apreventive effect. Shandong protected cultivation area are4×105hectares, accounting for about40%of the whole nation, but due to the widespread occurrence of gray mold, it has a seriousimpact on the yield and quality of fruit and vegetable crops, which exerts an unpredictable effecton the agricultural income.In this study, in order to monitor resistance of Botrytis cinerea in Shandong province, graymold samples are collected from the10cities of different protective area in Shandong province.Through the sterile separation in the laboratory,130strains of Botrytis cinerea are received.Chemical fungicides and more benomyl, procymidone treatment, diethofencarb, pyrimethanil,fludioxonil resistance levels are researched, as well as the preliminary studies of biologicalcontrol to facilitate rational drug use, avoiding drug resistance and multi-drug resistance, as wellas prevention of the disease provides a theoretical and practical basis.Through the gray mold pathogen isolated plant morphology and molecular biology research,the Botrytis cinerea conidiophores erect, bushy brown or light brown, smooth, top with1or2times branching, branching ends of enlargement, a large number of spores above clusters(unicellular, oval or nearly round, colorless or gray). Through the identification, the originalstrain is Botrytis cinerea fungus (Botrytis cinerea).By measuring130strains of Botrytis cinerea in10μg/mL carbendazim,5μg/mL ofprocymidone,1μg/mL of diethofencarb,5μg/mL of pyrimethanil,0.5μg/mL of five chemicalsfludioxonil frequency of fungicide resistance we can draw that, carbendazim resistant strains oftotal121, accounting for93.08%; resistant strains procymidone of88, accounting for67.69%;diethofencarb resistant strains109, accounting for83.85%; pyrimethanil-resistant strains117,accounting for90%; fludioxonil resistant strains73, accounting for56.15%.By measuring the EC50values of Botrytis cinerea, can be seen, the average EC50tocarbendazim is439.16μg/mL, the middle resistant strains accounted for65.5%, the highresistant strains accounted for31.03%, the average EC50to procymidone is4.03μg/mL,low-resistant strains accounted for52.38%, the middle resistant strains account for19.048%,there is no high resistant strains, the average EC50to diethofencarb is15.76μg/mL, low-resistantstrains account for68.09%, the middle resistant strain account for4.255%, high higherresisitance strains account for10.638%, the mean EC50to pyrimethanil account for17.19μg/mL,low-resistant strains account for70.21%, the resistant strains account for21.277%, high resistant strains account for8.511%, with an average EC50to fludioxonil is0.0367μg/mL, low-resistantstrains account for58.33%, and there is no high-resistant strains. It can be frequently used inapplications earlier carbendazim and procymidone which has reached a high level of resistance,but in recent years the use of diethofencarb, pyrimethanil, the most of them is at a low-level, andshould be used with caution, avoiding the generation of high resistance.Through tablet confrontation method to detect the biocontrol Bacillus subtilis Bacillusamyloliquefaciens reconciliation of inhibition against Botrytis cinerea, we can see that inBacillus amyloliquefaciens inhibition rate reached91.25%, while the inhibition of Bacillussubtilis was76.52%, as it can be seen in Bacillus amyloliquefaciens control effect is strongerthan Bacillus subtilis. So as a convenient and pollutive controlling method, it can be researchedin a more in-depth way.