Study On Fourier Transform Infrared Spectroscopy Of Vicia Faba And Armillaria

Broad bean and armillariella mellea have high nutritional value and medicinalvalue. In this dissertation, Fourier transform infrared spectroscopy combined withmultivariate statistics was used to study broad beans and armillariella mellea.Fourier transform infrared spectroscopy was used to indentify two kinds ofbroad beans which have white and green cotyledons color. The results show thatbroad bean seeds mainly contain starch and protein, the main component of seed coatis cellulose. The spectra exhibit differences for the two kinds of broad beans in theregion from1700to700cm-1, which were selected to perform principal componentanalysis, and yield accuracy rate of97.5%.The spectra of the fruiting body of armillariella mellea show differences in1800~800cm-1range. Curve fitting analysis results indicated the areas of secondarystructure of proteins were different. According to the differences, different armillariamellea species could be identified by Fourier transform infrared spectroscopy.The study shows that FTIR combing with chemometrics could identify broadbean and armillariella mellea by establishing infrared spectral database of thesamples.