Location Of Flax Resistance Gene In Flax

Flax(Linum usitatissmumL.)is Linaceae(Linaceae) flax(Linum) annual herbaceous plant fibers. Powdery mildew can harm the growth of flax, which is characterized by the onset of the plants will be covered with linen surface powdery white mycelium layer, the pathogen can infect all parts of the plant from the ground to the top, the more rapid spread of the disease, with the consequences of the decline came in advance for flax, lower production, economic decline.This experiment in the early by heilongjiang academy of agricultural economy Xue Yang based on the field disease-resistant material 9801-1 and infected Diane hybrid F1 and F2 generations of investigation, prove representative now completely disease-resistant F1, F2 generation of anti sense of separation ratio is 3:1. Prove that resistance to powdery mildew in 9801-1 is completely dominant, belongs to the nucleus genetic.This experiment is on this basis, the use of molecular marker technology, the use of high-generation backcross hybrids selfing 9801-1 and Diane get BC3F6 generation,were positioned flax powdery mildew resistance gene analysis. Test results obtained are as follows:Regular growth of flax field observation records, during the period of flax powdery mildew disease spores of flax powdery mildew fungus. Bacteria under the microscope to powdery mildew spores form, and completed the inoculation test in the field.First of all, I choose three kinds of molecular markers, SSR markers, ISSR and fixing the flax powdery mildew resistance gene analysis, validated analysis found no clear chain closely with resistance genes.So on this basis, the two parents to take a whole-genome sequencing materials.Sequencing to Lusitatissimum.m erge.fa as a reference genome. For ease of analysis,even as false Sequencing chromosome DNA library constructed two were obtained18.07 G the Clean data. After comparison of the sequencing analysis, the distribution of the reads in the longest 11 chromosomes(Scaffold) on. While the SNP, InDel, SV distributed annotation statistical analysis. Based on this data, there are differences in the sites of the primers were designed experiments were designed 121 pairs In Del mark, which covers the 11 chromosomes(Scaffold).By screening In Del mark successfully gained a resistance gene closely linked markers, InDel mark f2x4105, which is located ChrNew02(Section 2) on chromosome near scaffold145, realized flax powdery mildew resistance gene initial positioning.According to section now mapping and cloning of powdery mildew resistance genes related to this position temporarily out of the gene named Pm-Linum.To test the accuracy of the experimental data, I use 60 height backcross population with the aim to detect gene is not linked to the two marks, and found its exchange rate can reach 72%, while the target gene markers linked exchange rate was only 11%.