| The sequences of porcine type III interferons-PoIFN-X,!,-X3 were obtained from GenBank by BLAST. Specific primers were designed 1:0 isolate PoIFN-人 1,-人3genes from PK-15 cells. Sequence analysis was take打 out using software. A phyloge打etic tree of the IFN-入 gene family of different species defined the evolutionary relationship of various subtypes. PoIFN-?^l,-X.3 were then cloned into pcDNA3.1/inyc-His(-)B vector and expressed in BHK-21 cells, Westem-Blot analysis indicated 化at PoIFN-人 1 and PoIFN-X3 have 1 and 2 N-glycosylation si化s,respectively. Differential expression of the PoIFN-入 subtypes was detected in IB艮S-2and PK-15 cells in response to foot-and-mouth disease viras and pseudorabies virus.PoIFN-Xs significantly induced IFN stimulate genes such as MX, OAS and PKR,as well as IL-6 and TNF-a. PoIFN-X^s possesse过 weaker antiviral activity than PoIFN-al2 in both P民V/PK-15 and IBRS-2/FMDV systeIn this study, we determined the antiviral activity of porcine IFN-X,-co,-5families in 巧RS-2/FMDV,PK-15/PRV and Marc-145/PRRSV systems. The antiviral activity varies 江 lot i打 different subtypes and PoIFN-cd? exhibits the stronger inhibitory capacity against PRV and PRRSV, which is even higher than PoIFN-al2.We also characterized the activity of combination therapies with these types of porcine interferons against different viruses. When lU PoIFN-cos and PoIFN-5s was used in combination with lU PoIFN-a 12, i*espectivelys enhanced antiviral activity of10?200 folds was observed in EBRS-^/FMDV systems. Different ratios of PoIFN-Xs/PoIFN-a 12 Ksulted in various increasing times of anti-FMDV activity ranging from 5 化 500. Enhanced induction of some ISGs was detected by 艮eal Time PCR in IBRS-2 cells after the combined treatment of inteferons. What^s more,PoIFN-⑩ 1,-访2 showed 化e stronge巧 synergetic effects against PRRSV in Marc-145 cells. In PK-15/ PRV system, all combinations exhibited synergetic activity except the combinations of PoIFN-X3 with PoIFN-al2. |
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