Study On Evaluation Of Germplasm Resources Of Radix Paeoniae Alba In Zhejiang Province

Purpose:This paper takes the Chinese traditional medicinal materials Radix Paeoniae as the research object, Quality evaluation of science of Radix Paeoniae by morphological, chemical quality and genetic diversity, screen out the dominant population, provide the source for good breeding fine varieties of Radix Paeoniae.Methods:Collect Zhejiang Panan, Anhui Bozhou, Sichuan Zhongjiang, Shandong Heze, 12 populations of Paeonia lactiflora germplasm resources.Characteristics and microscopic characteristics of Radix Paeoniae, Radix Paeoniae and morphology of bud stem, leaf, flower and fruit were observed in different populations, the total paeony herbs in the determination of the effective substances of different populations, the fingerprint of Radix Paeoniae HPLC, and studied the genetic relationship between populations by DNA molecular marker technology on the basis of comprehensive evaluation, Paeonia lactiflora germplasm.Results:1 Through the various categories of biological characteristics of Radix Paeoniae, the medicinal properties and the microscopic characteristics can be seen from the comparison of Radix Paeoniae overwintering buds, stem epidermal spots much, leaf type, the carpels characteristic, the root shape, root skin color, fracture is exposed to the air for chromotropic, root central catheter group and ray arrangements can be the distinction between Radix Paeoniae group. According to the regional cultivation distinguish Radix Paeoniae tradition way group, because the modern transportation and information developed around the seedlings, mutual exchange and hybrid, have been very difficult to distinguish.2 Through the comparison of various categories of total effective substances of Radix Paeoniae, of which the total leaching of polysaccharide and the content of paeoniflorin in different populations exist significant differences between groups, may have some relevance to a certain extent between quality and biological characters of Radix Paeoniae. The total synthesis of effective substances, class I and class IV Radix Paeoniae populations has certain advantages in the whole, and class V Radix Paeoniae populations effective amount of material contained in the relatively low.3 To establish the fingerprint of Radix Paeoniae by HPLC, can preliminary identification of different populations of medicinal materials of Radix Paeoniae, compared with the standard fingerprint similarity is low, indicating that the chemical composition different from each other, which may be associated with white peony cultivars, growth environment, climate, cultivation management etc. Through the population genetic characteristics and genetic diversity analysis of preliminary, show differences in the gene DNA molecular marker technology from the molecular level detection of different populations between Radix Paeoniae, can lay the foundation for the research Daodite specific carry out of genuine medicinal materials in the next step, also provides the scientific basis for the identification of germplasm resources of Radix Paeoniae.Conclusion:In theory, through the comprehensive evaluation of the three aspects of ecology, chemical quality, genetic diversity and screening, can choose one to two dominant populations, but the interaction of genetic factors in reality because of the climate, soil and other environmental factors and their own, so that uniform result can not be good. Preliminary screening of integrated three germplasm results combined with Radix Paeoniae growth status under natural conditions, choose some of the advantages of good integrated performance populations:Dahongpao Gaoganhonghua, Yanzhidianyue, Hongyunliantian, Wulongtanhai population has a certain advantage on the whole, in the region can be given priority promotion.