Synthesis Of LDH @ SiO 2 Shell - Nuclear Nanocomposites And Its Application As DNA Vaccine For Newcastle Disease

Newcastle disease (ND) is a highly contagious, pathogenic disease which is caused by Newcastle disease virus (NDV), which causes great harm to animal husbandry, It has been defined as type A infection disease by International Veterinary Situation/The main symptom of ND is dyspnea, diarrhea, neurological disorders, mucous membrane and serosa hemorrhage. Vaccination is the principal measures to control Newcastle disease. Inactivated vaccine and attenuated vaccine is currently two main types of vaccine against Newcastle disease, although the immune efficiency is very good, but the protection period is not long, and there will be recovered powerful virulence. DNA vaccines can induce the humoral immune and cellular immune, but DNA vaccine also has shortcomings, that are easy to degrade, the amount of antigen expression is low. Using nano-materials as delivery carrier, can protect DNA from nuclease degradation, improve the transfection efficiency and targeting, enhance the immune efficiency.In this study, we encapsulated plasmid DNA vaccine (pVAXl-F(o)) as a model drug in LDH@SiO2-nanoparticles (pFDNA-LDH@SiO2-NPs). The formulation, preparation, physicochemical characteristics, stability of pFDNA-LDH@SiO2-NPs and antibacterial activity were evaluated. The results demonstrated that the pFDNA-LDH@SiO2-NPs had been produced under the optimization conditions with morphous regulation, good dispersion, the average particle size was 371.93nm, the zeta potential was+31.63mV, the drug loading was (39.66±0.45)%(n=3); DNase I digestion test showed that pFDNA-LDH@SiO2-NPs had the ability to protect DNA from DNase I degradation; antibacterial activity results showed that pFDNA-LDH@SiO2-NPs have obvious inhibition effects on the Candida albicans, Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa; The release behavior of pFDNA-LDH@SiO2-NPs test in vitro demonstrated that the release of pFDNA-LDH@SiO2-NPs could make plasmid DNA vaccine sustained release.ND DNA vaccine encapsulated in the LDH@SiO2-NPs had been assembled successfully. The pFDNA-LDH@SiO2-NPs could reduce vaccination times, increase the vaccination methods and enhance the immunological effect, which possessed a certain scientific significance and potential application prospects for Newcastle disease prevention and control.