Comparative Proteomics Study On Root Membrane Of Sugar Beet M14 In Salt Stress

Salt stress is one of the major abiotic stresses affecting plant growth, development, and productivity of crops. Sugar beet is a dicotyledonous plant of the Chenopodiaceae that has a high economic value. It is a crop of halophytic nature and can survive high salt conditions, thus is an excellent material for studying plant response and tolerance to salt stress. Sugar beet monosomic addition line M14 was obtained from the intercross between Beta vulgaris L. and Beta corolliflora Zoss., and it contains the Beta vulgaris L. genome with the addition of chromosome 9 of Beta corolliflora Zoss.. The M14 has retained some of these characteristics, e.g., salt stress tolerance and apomixis.Plant cells have many membrane systems which have specialized specific functions, including the plasma membrane proteins and various organelles membrane proteins, membrane proteins are communication interfaces in plant to perform cell exchange with the environment. Plant cell membrane protein in control many of the major cell functions, such as metabolites, ion transport, vesicles transportation, etc. It first to be hurt under salt stress, and the membrane proteins in plants play a crucial role in the process to resist salt stress, membrane proteins change will cause changes in cell morphology. So it has important significance to research on mechanism of membrane proteins under salt stress.We have already analyzed leaf differentially expressed membrane protein of sugar beet M14 line under salt stress. Thus, this test will use the iTRAQ technology study root membrane protein of sugar beet M14 line under salt stress(0, 200, 400 mM NaCl) to perform quantitative proteomics analysis. Compareing the mass spectrometry information and search the no redundant sugar beet database using Proteome Discoverer software.Analyze protein(p < 0.05), 73 differentially expressed proteins are defined as a ratio greater than 2.0 or less than 0.5.In order to further study salt response membrane proteins, according to the BLAST tools and annotation information in Uniprot as well as the information from relevant literature. Salt response membrane proteins are classified. Identification of differentially expressed membrane protein in leaves were divided, respectively is: transport(32%), Miscellaneous(10%), signaling(10%), stress and defense(8%), energy(8%), degradation(6%), transcription related(6%), metabolism(5%), biosynthesis(3%), cellular structure(1%), Unknown(11%). Results show that the transport proteins, signaling related proteins and Miscellaneous proteins account for larger proportion. Usually membrane proteins involved in ion, water and proteins transport and metabolism. We found that most membrane proteins located in the vacuole membrane, nucleus membrane, plasma membrane, Mitochondrion membrane, and miscellaneous membrane through bioinformatics analysis. These results suggest that membrane proteins can perceive salt stress and transmit them to regulate membrane fluidity and permeability, mobilizing various organelles membrane proteins work together to resist salt stress. These processes work cooperatively to achieve homeostasis under salt stress conditions. This research has set the stage for further understanding of the salt tolerance mechanisms in sugar beet.