Responses Of Soil Microorganisms To Nitrogen Deposition In Winter In Xiaoxing'anling Forest

Soil microorganism is an important part of soil, is considered to be an important indicator of the soil caused by the change of the evaluation of the natural or man-made interference. As the global nitrogen deposition increased, the effect of the global terrestrial soil microbial ecosystem become bigger, especially to soil microbes under extreme climate conditions response has aroused widespread concern. Therefore, how to study the nitrogen deposition changes in community structure in winter soil microorganisms under the background of urgent and necessary. This study in our country important forest-- Xiaoxing’anling Feng Lin national natural protection zone of control experiment, the design of the control(natural snow, no nitrogen added), zero snow is no nitrogen added, natural snow is n addition(5 g·N·hm-2·yr-1) and zero snow by adding nitrogen(5 g·N·hm-2·yr-1) 4 types of processing, using high-throughput sequencing techniques for measuring soil microbial community structure, reveal the soil microbial community structure of winter snow cover and the response of the dual effects of nitrogen application. The results of this study are as follows:(1) the soil microbial richness in response to nitrogen deposition is obvious, specifically for the trend of soil bacterial richness were decreased, that is to add nitrogen inhibited the richness diversity of soil bacteria. The diversity of soil fungi richness trend is rising, that is to promote the nitrogen addition on soil fungal diversity.(2) The response of soil microbial richness is obvious, particularly under different responses of soil bacteria and fungi by snow is consistent, richness in Xiaoxing’anling mountain winter snow was caused by soil microorganisms greatly increased, indicating high winter snow is effect on soil microbial richness is positive.(3) The determination results of soil microbial community structure in winter using high-throughput sequencing technology, soil microorganism in different snow conditions, different nitrogen levels in each phylums, classes, orders, families, genera and community composition showed significant differences.