Studies On Variations Of Silkworm Eggs Phospholipids And ExPerimental Fluorisis Larvae Phospholipids

eased on methods for preparing phospholipids from other organisms and tissues, the appropriate method for silkworm egg is achieved through optimizing the extractive solution volume, extractive time and times etc. The amount of phospholipids is measured by phosphorus molybdenum calorimetry and the compositions are analyzed by TLC (Thin Layer Chromatography,ThC). This thesis is studied on variations of silkworm, tussah-silkworm and en-silkworm eggs phospholipids and experimental fluorisis larvae phospholipids. Main results are as following:1.Among silkworm, fussah-silkworm and en-silkworm eggs, there are significant differences in the amounts of phospholipids. For 1 gram eggs, silkworm> tussah-silkworm > en-silkworm, and for 1 egg, they are reversed.2.The amounts of phospholipids of Polyvoltine race (5ansi), Japanese race (Xuesong), Chinese race (Huafeng) and European race (SaIisill) are 13.2534, 12.8858, 12.0164 and 11.1494 mg/g egg, respectively. The main compositions of phospholipids are phosphatidylcholine (PC), phosphqtidylethanolmine (PE) and phosphotidylserine (PS). Estimated by color?of I-he dots, the amounts of main compositions are different, PCES. There is no difference in compositions among silkworm races.3.After oviposition, silkworm diapause egg should undergo pre-diapause stage, dicipause stage, hibernation stage, critical stage and stage of organogenesis. buring protection for diapouse egg, the amount of phopholipids is decreased gradually until critical stage, and increased from critical stage to orgonogenesis stage. The amount of phopholipids during organogenesis stage is nearly equal to that of pre-diopause stage. It illuminates that phospholipids are main intermediate products in phosphorus metabolism. With prolonging egg age, phospholipds are decomposed slowly, and during organogenesis stage, more phospholipids63Qrs SyntheSiztd to m6at the demQndS from growin9 or9Qns.4.During 1-2 dQys Qf t6r coId-storQ96 QCid tr6Qtment, the Qmount ofphosphoIipids of silkworm 6ggs, which is from Bing1-2 to Ding1 embryostQ9es, is reduced signifiCQntIy. B6cQUse nerve ceII is QppeQring Qnd needslots of phosphoIipds to be decomposed to 96t QC6tyIcholine, the Qmountof phospholipids is the I6st in Din92 embryo stoge. Then it is increQs6drQpidIy in Wul embryo stQge. From Wu1 to Ji4 embryo stQges, it is rothersteQdy. ^nd it is increQsed suddenIy in Ji5 embryo stQ96. FortusSQh-siIkworm eggs, the amount of phosphoIipids i5 increQsed sIightIyfrom 8ingl-2 to Wu1 embryo stQges, becQuse tussoh-siIkworm egg isnon-diQpQUse eg9 Qnd needn't experience QctiVQtinq embryo 5tQ9e Qndlon9-time protection. 5ince Wu2 embryo stO9e, it is simiIQr to thecountcrport of siIkworm eg9.5.As the temperQture recedin9 rQpidly, Q pQrt of pho5phoIipids inbIuish-siIkworm-e9gs Qre decompos6d to adQpt Iow temperQture. ItresuIts thot the omount of phosphoIipids in b1uish-siIkworm-eg9s with nocoId-atorQ9e treatment iS hi9her thQn those with coId-storQ9e treQtment.There is no lineQr correlQtionship betwe6n the Qmount of phosphoIipid5Qnd one-doy-hotchobiIity for cold-storQ9e treQted bIuish-si Ikworm-e99s.From VQriQtions in phophoIipids durin9 coId-storQ9e tr6Qtments, we moyconcIude thQt the SQf6ty tim6 for bluish-siIkworm-e9gs cold-storQ9etreQtment is 6 dQys. CoId-storQ9e treQtments from 7 to 13 dQys, Qnoth6r= QIimentQtions in bIuish-siIkworm-eg9s Qre decomposed to tynthesizephosphoIipids Qnd it wiII I6Qd to reduce surviVQl rate of IQrva6 QfterhQtchin9. ^nd CoId-storQge treQtmehts more thQn l3 dQys, phosphoIipidsin membrQne Qre decompos6d Qnd it wilI IeQd to deQd ceII Qnd d6creQseon6-dQy-hotchability simultQneously. -6.There is diversity in the Qmounts of phospholipids for .VQriedqUQIity siIkworm eggs: FertiIized eggs > non-fertiIized eg9s >deQd6 4e99s. For tussQh-siIkworm e99s: Fertilized e99s De non-fertiIized e9g5.7.For SQme vQriety, siIkworm eg9s, which Qre mQde in Qutumn, hQvemore pho5ophoIipid5 thQn those of in sprin9. It mQybe r...