Equiine Arteritis Virus: Hemagglutination And Its Antibody Detection From Entry-exit Horse Sera

The hemagglutininablity of equine arteritis virus (EAV)Burucys strain cultured in RK- 13 cell was tested with erythricytes from different animals at 40C,room tenperation and 370C.HA was observed at all temperatures with mouse and chicken erythrocytes but not with those of cattle,horse.rabbit.guinea pig, and goose. The HA activity was enhanced after treatment of virus material with Tween 0 and ether.Compared with the treated EAV with Tween-80/ether and the untreated virus,the HA titers of treated group were 4- and 8- fols higher tha the untreated and the HI pattern of the treated virus were more obvious.The optimum conditions were as follows:pretreatmcnt of virus materials with Tween-80 at a final concentration of 0.06---0.125%(V/V)for l50 mm ,and treatment with ether at a concentration of 50 O/ (V/V) for 5 1 5mm in ice bath with continuous shakings.