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Genetic Analysis Of Successive Two-way Selection In Offspring Of Dwarfing-sterile Wheat Population

Posted on:2001-09-30Degree:MasterType:Thesis
Country:ChinaCandidate:Y WangFull Text:PDF
GTID:2133360002950392Subject:Genetics
Abstract/Summary:PDF Full Text Request
he selected ti81tt81s'dlveGence ofsubpopulatlons,Winoh were formed in four generations of successive two-way selection of dwhg-tenle wheat population,was examined by quantitative analysis t Mparent level,andthe genetic stFI.---tLlte of*the--tLlte fourth genefstion ws sNdled by RARAPD tecMlques t molecular level. 刀吐 results ofqUanlqUanlive analaniysls are as follows.All he selectCd汀印s hadhad exPe咖d Pro囚旧ss.he deg卜e oflt expressedvanously indifferent genelllons,iorem ti81is,and two directions ofonetrslt;howeveLgeneM correspondence was In dl&rein Rmlityo艳p血g.皿 s咖icantdlfferences were fomd In mostpnnclpal coMoMnt genetic d灿m田.It showed that血selection hdmde POpulstions di肚remiated.At he same tUne,he genetc vanation was mamtuned. 11 out of 50咖itrny pnmers,Mob produced 138 ishnctive bands In total,were 咖ptCd n冰 RAPD analysis.Based onthe amplified bands,someparameters of genolc v叼ation were estlmMd by using the gene hquencXThe result revealed 山用:T比 selection hd effects on the selected populstlons.刀岭 hi-direction ofgene hquences developed towards…们differentM叮s,compared ith unselected populations(US).刀帕 ercentages ofpolymorphlc locus,thehe肋wgosltles of*theles selected po卯lations we比 lowerthan the unselected populations(US); 彻weve呻饲antgemtlc vanatlons still existed In临 selectedpopul劝ons.All the gene frs叭nces were nearo.4,the perce腑ges ofN帅orpmc locus(>70%)and the he加出gosities were at hi吵 level;he genetic dl地renlatlon删 small among all subpopulMons.n把 relative gene dl征remlatlon index(Gst仰d he ixation index (Fst)of he fourth generation were 0.1220 nd 0.1343 respected.he Gst of mixedt populstions was 0.1274,which ws similar ith脓 former.The劝ove showed that奇 most gene0c vanatlons existed m iinun-populstlon and some existed In among- popolatlOO. he results of mlnunuxn genetic distance,standard genetic distance and maximum genetic disinuce聊eared:Din<Ds<D(Ds&Dv h的 closer relatiomhip. RAPD ge砒tic distance andthe pnnclpal compo肪m distance h劝 similar cN幻a results exceptthe location ofW4andM4 In of四忆rfigure.地 correlstion ofboth dis柏nce(Dv-D)waso.4144whichamountedo.10s咖icantlevel.Itwas shown伽 the刊们d劝mce were In agreementic some degree....
Keywords/Search Tags:Dwarfing-sterile
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