Genetic Analysis And RAPD Markers Of Chipping Color In Diploid Potato (solanum Tuberosum)

The potato chip processing is confronted with a basic problem how to obtain satisfied chipping color. Screening qualified raw materials is essiential to resolve this key problem. This article systemically introduces research history, principle, influencing factors, three breeding strategies, and new advancement of molecular biology on potato chipping color. It is very important to develop desirable genes that control chip color from wild diploid species in potato breeding programs. To identify?the new germplasm on chip color, analyize genetic components and investigate phenotype of chip color will be very helpful to formulate a rational breeding project. The molecular markers of chip color genes in suitable populations provide sound basis for further research on markerassisted-breeding, locating and cloning chip color genes. Three different populations of diploid potato were employed to study chip color in this experiment. Seven different processing traits among the three populations were analyzed. According to a certain program of chip processing, all genotypes in three populations were evaluated strictly on their chip colors. The molecular markers of chip color genes were studied with the method of Bulked Segregant Analysis(BSA). The main results as follow:1.The integrated analyses were conducted with chip color assessment and processing traits in population level. The results showed that GG population was good for chip color breeding and the genotypes with light color can be screened easily from this diploid population.2.According to the evaluation of chip color and comparing with the chips of Atlantic and KeXin 1 (CK), sixteen individuals from the diploid potato with light chip color were preliminary screened. Based on comprehensive assessment, nine individuals of diploid potato with good qualities of chip processing were finally screened.3.It was concluded from the frequency distributions and the segregation4ratios of chip color values in three populations that the frequency distributions ofchip color values in three populations derived signiflcantly from normaldistribution so that potato chip color may be controlled by at least tWo pairs ofgenes.4. TWo MD markers, OPH05,,, and OPW04,,,, were found to be linkedwith chip color genes by screening 260 random primers.