Study Of Differently Foreign Gene Effects On Bollworm Resistance And Heterosis In Upland Cotton (G. Hirsutum L.)

The bollworm resistance was systematically analyzed about three kinds of transgenic cotton, Bt transgenic cotton line GK12, (Bt+CpTI) transgenic cotton line SGK9708, and API transgenic cotton line Sukang 2534. The relationship between the content of Bt insecticidal protein and bollworm resistance was studied with two Pt cotton lines, GK12 and SGK9708 as well as their respective F1 generation. The contents of Bt insecticidal protein and gossypol in the Fi generation of GK12 crossed with high-gossypol cotton line HG-BR-8 and with low-gossypol cotton line 471219 were utilized to analyse the inner relationship with their bollworm resistance. The transgenic cotton varieties GK12, SGK9708 and their correspondingly receptable parents Simian 3, Zhongmiansuo23 were served as the research materials, and the foreign bollworm-resistant genes?influences to the receptable parent characteristics were studied. The Fi generations of the coventional cotton line 5121 with transgenic cotton lines GK12, SGK9708 and the corresponding receptable parents Simian3, Zhongmiansuo23 were used to analyze the Bt gene (Bt+CpTI) genes respective influences on transgenic cotton hybrid characteristics. The three bollworm-resistant genes (Bt Bt+CpTI API ) had obvious resistance to bollworm by laboratory bioassay, and the resistance showed evidently temporal and spatial distribution. The resistance of Bt gene was inferior to that of (Bt+CpTI) gene before the blooming and boll set stage, and better than that of (Bt+CpTI) gene after the blooming and boll set stage. The resistance of API gene was not as good as that of Bt gene and (Bt+CpTI) gene in every growing stage. In the blooming and boll set stage, the resistance of API cotton? vegetative organ was better than that of Bt cotton and (Bt+CpTI) cotton. But the resistance of API cotton in reproductive organ was not equal to that of Bt cotton and (Bt+CpTI) cotton. The F generation of the transgenic cotton in the resistance tendency was similar to the correspondingly transgenic parent. The resistance of the Bt cotton was better than that of its F1 generation in every growing stage. In blooming and boll set stage, the resistance of Bt cotton vegetative organs was better than that of F1 generation, and the resistance of Bt cotton reproductive organ was inferior to that of F1 generation. The resistance of (Bt+CpTI) cotton was better than that of F1 generation before the blooming and boll set stage, and was not equal to that of Ft generation after the blooming and boll set stage. The resistance of (Bt+CpTI) cotton organ was not better than that of its F1 generation. It had the trend to decline in the resistance of Bt cotton Ft generation. The resistance of (Bt+CpTI) cotton in F1 generation was better than that of its transgenic parent. The expression of Bt insecticidal protein was better in Bt cotton than that in its F1 generation, and better in (Bt+CpTi) cotton F generation than that in its transgenic parent. The content of the insecticidal protein was directly correlated with the bollworm resistance in transgenic cotton. The resistance of the F1 generation of GK12 with high-gossypol cotton line HG-BR-8, low-gossypol cotton line 471219 tended to deline. The expression of resistance in laborary bioassay was GK12> (HGBR8*GK12) F1> (47 1219*GK12) F1>HG-BR-8>471219. The content of Bt insecticidal protein in GK12 was more than that of the hybrid F1 generation of GK12. The content of insecticidal protein...