| Hepatitis C virus(HCV) is one of the most widespread viral disease infected human health. Different from the other hepatitis virus infection, it can cause higher proportion of chronic disease, even higher than 80%. So it has bigger harmfulness. Up to now there is no effective treatment available for chronic hepatitis C, the prevention and control of hepatitis C by using safe, economical and universal vaccination is highly effective. In this research, tomato plants will be transformed to express CE and NS3CE genes of HCV. People eat the transgenic tomato and need not to cook it, meanwhile they can get immunity. NS3 and CE genes of HCV were selected to be target genes according to relevant data. NS3 and CE genes were obtained by PCR amplification. The Serminal of CE cDNA fragment was linked up with the 3erminal of NS3 eDNA fragment by a oligonucleotide linker Serrolyer to form a chimeric gene NS3CE(2.Okb). The gene CE and chimeric gene NS3CE were recombined with the expression vector pBIl2l respectively, and constructed two plant expression vectors: pBICE and pBINS3CE. Leaf disks were cut from sterilized leaves of hongshu 5? (Lycopersi con esculentum), inoculated with Agrobacteriuw twnefaci ens EHA1O5 containing CE or NS3CE. Plants were regenerated on selective media with Kanamysin. The presence of the CE or NS3CE gene was confirmed by PCR and Southern blot in plants surviving sections. RTCR results showed that CE and NS3CE genes have been expressed at the transcription level respectively. Vaccine antigens cloned into edible transgenic plants are a promising new delivery system for oral vaccines. Such vaccines could be safe, inexpensive and multicomponent. In this research, the author wishes to do some foundational and valuable work on HCV edible vaccine. |
