Studies On The Immunoassay Of Fipronil

Fipronil(F) and one of its analogy(FH)were adopted as haptens0 The former was coupled to carrier protein by both EDC and GD, and the later was coupled to carrier protein by active ester. These protein-hapten conjugates acted as man-made antigens. Immunity experiment results indicated that FH2934,FH2962 and F2895 elicited special antibodies to Fipronil, and the titers are 25,600,12,800 and 12,800 ,respectively, when tested by indirect ELISAO The most tow limited quality is as low as 0.2 ppb.The effect of several physicochemical factors on the immunoassay for fiprotiil was studied and the results were used to optimize the assay.The immunoassay was finally developed based on the FH-BSA polyclonal antibodies and employment of a homologous coating conjugate. The optimized enzyme-linked immunoasorbent assay ,performed in a high salt concentration buffer with bovine serum albumin(0.l%),and without Tween 20, has a fipronil130 of 17.95ppb in distilled water matrix, and 16.8ppb in field water matrix and4the lowest detection limits of 0.06556ppb and 0.03620ppb respectly. The tolerance of this fipronil ELISA to acetonile and tetrahydrofuran is better than to4acetone. The OD values of backgrand is higher when work solution cobtainsorganic solvent...