| cDNA microarray consisting of 1330 unigenes derived from rice endosperm cDNA library was applied to monitor the transcript abundance of different tissues ( root, stem and leaf) in rice line Zhongzao 21.The results demonstrated that microarray could be used to monitor the expression levels of thousands of gene in different tissues simultaneously.By analyzing the results of hybridization we got one tissue-specific full-length cDNA fragment (H012d03), which had none, trace and intensive expression level in tissues of root, stem and leaf respectively. Northern blotting confirmed the results.Electronic PCR results suggested that :his gene lie in the structural gene of 23S rRNA belong to rice chloroplast genome. No similar rice EST had been found in the public database.Some research has proved that there was one intron repeated gene encoding I-CPAI protein in 23S rRNA of chloroplast genome of green alga. The gene can transcript into mRNA with poly A tail and encode protein with bioactivity. In rice, we found it is the first time in the world that in the structural gene of 23S rRNA of chloroplast genome, there also have the intron repeated gene could transcript into mRNA with poly A tail.Meanwhile, Another cDNA microarray with 4370 unigenes from library of rice endosperm and leaf tissue was also used to detect the expression level of mRNA from stem tissue of rice treated by water and plant hormone ABA. Results showed that the expression levels of 5 genes were depressed by phytohormone ABA. Reverse Northern blotting confirmed that one of the 5 genes (H024g06) was really depressed by ABA. Bioinformatics analysis showed that the gene H024g06 is the same ascytochrome C gene. Previous researches revealed that cytochrome C gene was associated with such stress response as drought and coldness, while ABA could induce the stress response of plant. Therefore our results suggested that the gene cytochrome C may have certain function of mediate during the stress response induced by phytohormone ABA.
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