Studies On The Inheritance And Expression Of Transgenes And Phenotypic Variations In Transgenic Cry1Ab Rice (Oryza Sativa L.)

The linkage of crylAb and gus was verified by PCR, and the inheritance fashion of transgenes was analyzed by GUS bioassay in the transgenic progenies of four japonica varieties, Nipponbare, 93Van, 91RM and Zhong 8215 transformed by Agrobacterium-mediated method, hi addition, the ability of spores to transmit foreign genes, the behavior of CrylAb space-time expression, and the resistance to stem borers of transgenic plants were evaluated. The RVA profiles of several representative of progenies of the transformed plants were tested. The main results were as follows.1. Foreign genes, crylAb and gus, are linked in most of the transgenic plants. In the progenies of transgenic rice, the ratio of GUS positive plants, in most cases, was significantly less than 3:1, which is expected for a single dominant gene controlled trait.2. The competitive ability of pollens with transgenes is significantly weaker than that of normal ones without transgenes, which may be the main reason for the non-Mendilian segregation of transgenes. No similar phenomena were found for gynospore with transgenes.3. There is an order for the absolute content of CrylAb in different organs of a plant. The highest content is in the blade of flag leaves, the second in spikelets, and the lowest in roots, at flowering stage. The order from high to low is blade leaf>spikelet>middle sheath>middle stemMow sheathMow stem>root. But there was no any order for the CrylAb relative content to the total soluble protein. It is showed there was no trend for the expression level of CrylAb at different growing stages.4. The resistance to insect of transgenic plants hi laboratory is accordant with that in field, so insect-resistant plants could be identified through field selections.5. Silence of the gus was found in one transformant of 93Van, while silence of the crylAb was found hi transformants of 93Van, 91RM and Zhong 8215.6. Significant variations of RVA profiles were found among transgenic riceprogenies and their corresponding parents, somaclonal variations were the main causes of the kind of variation.