Screening And Identification Of CDNA Clones Of Differentially Expressed Genese Involved In Resistance Of Rice To Bacterial Blight By CDNA-Microarray Technique

The mutant SL3751 of the partial deletion of the rpfC gene of Xanthomonas oryzae pv. oryzae (Xoo for short) is a potantial biocontrol strain which could induce the rice (Oryzae satira) to produce the resistance to the bacterial blight caused by the wildtype strain 13751. With the eDNA micrroarray technique, eDNA clones of the 10 differential expression gnes of the seeding of the rice Jingang 30 before the tiller were screened, and further verified by Northern hybridization.The foreign cDNA fragments of four of the differential cDNA clones were sequenced.The results of analysis of the nucleiotide sequences displayed the four differentially espressed genes: cytochrome b5 gene,aquaporin gene,25SrRNA gene and a gene homologous to the gene encoding a hypothetical protein in Arabidopsis thaliana.With the exception of cDNA fragment of 25SrRNA gene, cDNA fragments of the rest of three differentially expressed genes were all in full length. As compared with treatments induced by inoculation of 13751 and the control , within the rice induced by inoculation of the strain SL3751 the expression level of the cytochrome b5 gene was lower than that induced by inoculation of the strain 13751 ,but higher than that induced by the control treatment. Expression levels of both the aquaporin gene and the gene homologous to the gene encoding a hypothetical protein in A.thaliana were highest, but the expression level of 25SrRNA gene was lowest. According to the aforementioned results,it might be deduced hat the resistance level of the rice to the bacterial blight caused by Xoo would be enhanced by the proper regulation of the expression level of these genes within the rice.