Study On Green Foxtail Diversity And Gene Flow Of Foxtail Millet(Setaria Italica (L.) Beauv) Resistance To Herbicide

Gene flow between cultivated and its wild relatives and genetic diversity of its wild relative are key issues of the potential risk of the use of transgeneic crops. This study analyzed the genetic diversity of green foxtail(S.viridc), yellow foxtail(S.glauca )and foxtail millet(S.italica) disturbed in the North of china using twelve allozyme (21 loci) ,such as AATS ADHS AMPS DIAS GDH.. IDHS MDHS ME~ PGD, PGI5 PGMS SKD. The test of gene flow between cultivated population of foxtail millet and its wild relative, green foxtail, was carried out in the field, using a dominant sethoxydim resistant line as a pollen donor and a population of green foxtail as a recipient. The main results are summarized as follows: 1. Characters of genetic diversity are followed Three allozyme loci AAT-3, PGD-1 and PGD-2 are monomorphic. The IDH-1 was silence in S.glauca but it was active in S. itlica and S. virids. Genetic diversity of green foxtail was more than that of most of other annual primary self-pollinated plant. The yellow foxtail was complex. Genetic variation in intraspecies of green foxtail from different region of North china is more than that of interspecies Sitlica and S. virids. Cluster show that genetic variation of green foxtail in different region is related to its ecology region. 2. Since genetic relationship between green foxtail from different region and foxtail millet resisted to herbicide is different, foxtail millet, which is relatively far away from green foxtail in local region as for genetic relationship, resist to herbicide is popularized in order to decrease the ecology risks. 3.This study found that the combined effect of H202 and GA3 could increase germ i nation rate of green foxtail to an idea degree. Only the effect of H202 could break out the dormancy of green foxtail from Huaian and germination rate was beyond 60%, but it could not increase germination rate of green foxtail from Salingzi to the same level. This study also found an ideal way to identify green foxtail resisted to herbicide through watching shoots trait which was seed germinating four or five days on mediate with certain dose of herbicide. 4.The results of gene flow test showed that: the pollen of foxtail millet could pollinate the green foxtail up to 60m. The highest frequency of gene flow was at 0.03m, reached 1.14% in average and 1.68% at the most. The frequency decreased with distance increasing. A sharp decrease of the frequency took place within 0. 5m from pollen donor plant. Generally, the frequency of gene flow became 0 at 20m, but it was possible that pollens dispersed from pollen donor sources up to 60m in some direction. The wind direction is the major climate factor influencing the frequency of gene flow. There was two times difference in frequency under two contrasting directions of wind. The variation of gene flow between Huaian and Salingzi was not notable.