RAPD Analysis Of Genetic Structure In Eucalyptus.grandis Populations

Leaf samples collected from 11 Eucalyptus grandis provenances population introduced from the geographic range of Eucalyptus grandis in the North of QLD in Australia are analysed for RAPD variation. The genomic DNA is extracted from 80 individuals leaves with 20 familes using modified CTAB. The analysis of the genomic DNA which amplified by 20 arbitrary 10-base primers obtained from 186 10-base primers reveals 201 amplified loci with 89 polymorphic loci and the proporation of polymorphic loci is 44.28%. Genetic variablity indexes are determined using 201 amplified loci including proporation of polymorphic loci (P), diversity coefflcient( DC), proporation of diversity coefficient( PDC), Nei抯 unbiased genetic identity (I) and Nei抯 unbiased estimates of genetic distance (D) .Genetic analysis of 80 individuals from 11 populations of E.grandis shows there is highly variation in genomic DNA with P values at 44.3%. The provenance of 18591 was the most one with 39.5% at P values, so is 18594 with 3 6.7%. The variability is high in population with an average of 0.6427 DC values, 7.27% PDC values and with the average of 0.1664 unbiased estimates of genetic distance. The dendrogram using two different methods shows that a high similarity is existed among populations of 18180, 18591, 18595, 17562, 18590, 18592and18594, butthereisa lower similarity between 17857 and the other populations. The study provides a scientific foundation on early selection in grandis populations.