Genetic Analysis And Localization Of Gene Relating Disease Lesion Mimic Of Rice

With more than 50 years development, genetic markers experienced morphological markers, cytological markers, biochemistry markers and molecular markers. The rapid development of molecular markers had become the important tool of genetic manipulation and plant breeding. Because of its rapid and simple, olecular markers were used to screen the linking markers, structure the genetic map and assistant plant breeding abroad.When no evident adversity, injury or pathogen infection, plant can form spontaneity cell death, forming necrotic spot in the leaf of plant. The symptom was very resemble to the spot formed by pathogen. This kink of mutant was named disease lesion mimic. The mutant provides a useful way of identifying the events that trigger HR and studying the steps that culminate in cell death. A new rice disease lesion mimic was found Cheng Zhukuan in 1998. It was controlled by concealed odd gene after the primary genetic analysis.The mutant SplSOl being female parent hybridized with 02428 and structured the segregation population. It was analyzed by means of SSR and RAPD technique in this study to find the molecular markers of the mutant gene.1. Through the mutant was gone to be morphology identification, such as non-bacteria cultured, to be shaded, to be inoculated with rice blast bacteria and dyed with trypan blue ,the mutant was assured preliminarily that it was not induced with pathogen, had thing to do with cell death, had aggrandizement trend with antagonism of rice blast and was controlled by ray.2. 190 pairs SSR primers were used and RM126 and RMS 10 locating the area of chr.Sshortarm centromere were linked with the mutant. The distance of the two markers and mutant gene were 2.9cM and 4.3cM separately. Disease lesion gene was a new gene named Spll2(t).3. 500 RAPD primers were used and OPI11 and OPL03 had steady difference segment among the parents and population by 6 times repeat experiment. The difference bands linked with the mutant and the genetic distance of the two markers and mutant gene were 0.6cM and 5.6cM separately. So OPI11-555 and OPL03.944 became the RAPD markers of the mutant gene.