Eliminated The Non-specific Responds Of The Cow Tuberculosis In PPD-ELISA

Obtained gum chromatography antigen 1, gum chromatography antigen 2 and immune-affinity antigen make use of the immunology methods of the gum chromatography and immune-affinity chromatography to handle to PPD antigen and compared the specify of antigens in this experiment, at same time absorbed non-specific antibody with the absorption antigen of M.phlei. It proved that PPD immune-affinity chromatography antigen is the best antigen. It is prominent difference when absorbed the serum with other types specific antigen.Make sure of the best method of ELISA examination at the same time in this experiments. Examined 141 heads of cow with tubercles allergy positive reaction and 124 heads of cow tubercles allergy negative reaction, This experiments achieved the purpose for eliminiate non-specific responding through eliminated interference of other types specify antibody such as M.phlei and strengthen the antigen specify. This experiment is the importance theories basis for the cow tubercle ELISA examination.