Studies On Micropropagation Procedure And Influence Factors In Rabbiteye Blueberry

Using Powder , Choice,Primer, Dellite, Balduin, Bluebelle, Gardenblue as plant materials and the micropropagation procedure of rabbiteye b\uebeny(Vaccinium ashei Reade) and several factors were studied . It was discovered that MS and WPM are more effective than Anderson and Knop and good medium were established successfully. The results also indicated: supplementing the induction and subculture medium with NAA, 2ip benefited the induction of blueberry buds, but 2ip maintained the ability of differentiation. Plant source especially genotype played no role on the bud differentiation of long-term culture; Different hormones (NAA,2ip and ZT etc.) affected the buds differentiation frequency. Among them, 2ip increased the frequency of bud formation, increased the frequency of the bud differentiation frequency; In subculture stage, we found that 30001ux, 40001ux light bud formation. In autumn, the explant which was pre-handled by low-temperature could enhance intensity was better than others; supplementing the root medium with IBA or NAA increased the formation of root, but IBA was the better one.