Construction Of Targeting Transgenic Vector For Silkworm And Study On Inactivity ChiA And CP Of Baculovirus To Express GIL-3

With the growth of economic and technology, it's possible to use the silkworm as a bio-reactor for production of heterologous protein. There are two main ways to produce interesting protein by silkwrom, the baculovirus expression vector system (BEVS) and transgenic silkworm.We coloned BmNPVsu IE-1 promoter, 1.2kb and 0.5kb gene of fibroin light chain from the 5th exon to the 7th exon to construct the transgenic silkworm vector. By means of insert report gene GFP which is downstream of IE-1 promoter in the fibroin gene we have coloned, it's possible to get the single copy gene of trasgenic silkworm which will spin silk and express the gene continually.We construct the vector pSKC-P-C which has Polh gene between ChiA and CP. The vector was infected BmN cells with BmNGIL-3 so that the ChiA and CP are out of function. The recombinant virus NdBmNGIL-3 we gained has showed that the expression time of GIL-3 was prolonged and the polh and GIL-3 was detected in BmN cells, accordingly, recombinant virus could be infected silkworm by eaten.