The Study Of Inhibitory Effect On Tumor By HTERT Based DNA Vaccine

DNA vaccine is one of the widely studied strategies in the field of immunotherapy for tumor. After successfully applied in the treatment of several human cancers, it turned out to have great potential value in the fight with tumors. In the study presented here, human telomerase reverse transcriptase is selected as the interest gene to generate a DNA vaccine. The effect of the vaccine on tumor growth is thoroughly discussed in the C57BL/6-Lewis lung carcinoma mouse tumor model.Human Telomerase is a tumor associated antigen whose expression level is up regulated in more than 85% of all kinds of human tumors. A large amount of effort has been focused on the finding of effective telomerase inhibitors that would repress telomerase activity thus limit the growth of human tumor cells. Here we applied a different strategy in which the catalytic subunit of human telomerase complex is selected as the target of a DNA vaccine. In this case, the specific immune responses are activated in the mouse immunized by the vaccine and the growth of the tumor cells planted to the immunized mouse is retarded or suppressed. The preliminary data show the effectiveness of the expression of the vaccine in vivo. The Lewis lung cancer cells grow slower in the immunized mice than that in the untreated ones, which is significant in statistical analysis in first several weeks after the challenge of the cancer cells to the immunized mice. The survival rate is also increased. The GM-CSF, a factor that enhances the immune responses, and hTSF, a synthetic angiogenesis inhibitors are used independently or combined to treat the mouse. Much more tumor suppression is achieved in both cases, while there is some difference in the extent to which the tumor inhibition effect is enhanced. Moreover highest survival rate is achieved when both factors are applied for the immunized mice. The safety of these vaccine and factors are also evaluated in the experiments without any obvious side effects observed. Collectively, the results from this research implies the potential effectiveness of the telomerase DNA vaccine with regard to the treatment of cancers and the sandwich like treatment on the cancer might give the best outcome.Master Candidate: Lv Yunzhe Major: Molecular Immunology Supervisor: prof. Li Jingpeng prof. Ren Darning...