| A Dot-PPA-ELISA method has been developed for the detection of anti-Salmonella antibody in pigs. Following work has been done for the establishment of the antibody detection method. Different kinds of Salmonella antigens have been tried and an improved heat-extracted antigen was used as the diaphragm antigen. The optimum condition of Dot-PPA-ELISA was determined . The antibody positive standard of Salmonella infection was established as 1:32,and the positive standard of protecting pigs from Salmonella infection as 1:512. The high specificity of Dot-PPA-ELISA was proved by the specific blocking test,and also by the cross-reaction test in which the diaphragm didn't react with the antibodies against Pasteurellosis,Streptococcosis,Colibacillosis,Chlamydiosis,HCV,PPV,Brucellosis,PRV and foot-mouth disease. The diaphragm has good sensitivity and could detect some Salmonella-positive test serum which has been diluted to 1:2048. Stored at 4 for at least 6 months or at 10-25 "C for 4 months,the sensitivity and specificity of the diaphragm didn't change,so it has good stability. This study indicates that the Dot-PPA-ELISA was a convenient,rapid,sensitive and specific method for the detection of Salmonella antibody. Otherwise,all of the materials used in Dot-PPA-ELISA can be standardized,so it's fit for the surveillance of Salmonella antibody in herd and for the epidemiologic investigation of Paratyphus swine. |
PDF Full Text Request