Pathological Study On Experimentally Leucocytozoonsis Caulleryi In Chick

300-day-old layers were experimentally infected with Leucocytozoonsiscaulleryi by co-habit. The pathomorphology , biochiemical indexes and morph-structures of the Leucocytozoon Caulleryi were studied. In infected chickens,the first changes is hyperemia in heat,liver,spleen,lung,kindey, entestine, muscle etc. Then hyperemia,hemorrhage and necrosis become sevevity. Especially the hemorrhage of kidney and ovarium and the coagulation necrosis in liver and spleen were obvious. In addition, there were much of epithelioid cells, plasma cell, lymphocyte, eosinophil posed cells tuber in organs, especially in liver and spleen. At the same time , there are Megaloschi-zont of Leucocytozoon caulleryi in organs. Observation through electron microscope, the mitochondria in the cells swelled, the crest became short or disappeared, and ground substance emptied, also nucleous and karyotheea disappeare, chromatin get together and appeared indention. About biochiemical indexes, the results were as follow: RBC and Hb were decreased post inoculation of L. Caulleryi, and decreased significantly in the later stage (P<0. 01); Glu was increased; serum LDH and AMS level were increased post inoculation of L. Caulleriyi, and decreased significantly in the later stage (P<0. 01) ; serum ALT level were increased in the easly stage and decreased significantly in the later stage (P<0. 01); T PROT and ALB concentration were decreased (P<0. 05), Ca level were increased. About morph-structures of Leucocytozoon Caulleryi in chick, results evealed that:The merozoites under light microscope, Leucocytozoon caulleryi appeared to be round, possessed a red round nucleus. The gametocytes appeared to be round to oral. The macrogametocytes possessed a dark red small nucleus, the43diameter is 16. 65 ×15. 79um;The microgametocytes possessed alarge nucleus, the diameter is 11.27×11. 1Oum. The macrogametocytes were also easily distingnished from microgametocytes by their dark stainning appearance. Underelectron microscope, the merozoites appeared to be round to oval and were surrounded by two clearly marked membranees, i. e. a thin outer and a thick inner membrane .The merozoites possesed a large round to oval nucleus, a small nucleolus.The micro-and macro-gametocytes were surrounded by 3-layered cleary marked membrane as in leucocytozoon sabrcizesi.But in the mature gametocytesof L. caulleryi only 2-layered membranees were seen. The forepart gametocyte of leucocytozoon in red blood cell appeared to be round to oval in shap and were surrounded by 2-layered membranees and possed a large round to oval and dark stainingnucleus.