Manufacture Of Trichogramma Carrying Bacteria And Evaluation Of Effect On Controlling Asiatic Corn Borer With Them

This article studied the manufacture of Trichogramma carrying Bacteria,by screening T.species and strains which have the parasitic dominance to Asiatic corn borer eggs and screening pathogenic species which have the control dominance of Asiatic corn borer, and by study the effective method of the conjunction of Trichogramma and bacteria . We used the method under lab 梥emi-natural condition梟atural condition to screen Trichogramma species and strains which have the parasitic dominance of Asiatic corn borer eggs. But we used the method of Semi-natural condition?natural condition -lab to screen pathogenic species which has the control dominance to corn borer .This method is different from routine method. This new biological control technology not only increase the control effect of Trichogramma, but also provide convenience for using bacteria.Because the commercial Scale production technology of minute eggs such as Corcyra cephalon and Sitotroga Cerealella hadn't been finished in China, so we screened the Heilongjiang strain of T.dendrolimi which could be commercial Scale producted as the T.species and strain which had the parasitic domimnance to Asiatic corn borer .It potencially parasitic capability could reach 17% and parasitic rate was as same as T.ostnmae species and strains in semi-natural condition.The result of the study indicated that Bacillus thuringinsis, especially Variety Beijing2 had the infection dominance to Asiatic com borer. In semi-natural condition, the rate of dead pests reached 17.1%,which significantly higher than any of the others .Under natural condition , the rate of dead pest reached 12.4%,Although, variety Beijing2 and variety Wuhan were significantlyindifferent(p<0.01),but variety Wuhan is superior to other species .Under natural condition , Beauveria bassiana lives long time .when condition is suitable , it'll infect ,even lead to epidemic .So in the long run its control effect is better than Bacillus thwingiensis . Green muscardine couldn't infect Asiatic corn borer, so we think it isn't suitable as one kind of bacteria for Trichogramma to carry to control Asiatic corn borer. We could evaluate the control effect of Trichogramma carrying bacteria under nature condition. We viewed that Trichogramma carrying Bacteria can increase the control effect over 10%,the highest was 15.2%. Bacteria which Trichogramma carried didn't affect the parasitism of Trichogramma .So the use of Trichogramma carrying Bacteria is a new perfect biological control technology.The conclusion also demonstrated the best method in the four methods by which Trichogramma carried Bacteria is using the utensil with which releasing Trichogramma. The utensil didn't affect eclosion rate and parasitic rate of Trichogramma. Furthermore, this method made Trichogramma carry Bacteria 3.5xi06,much more than any other method made .So the use of utensil with which releasing Trichogramma are beneficial to the conjunction for Trichogramma and Bacteria.