| Common wheat was the most important crop in the world so its quality can crucially impact on the world crop market. But cultivar wheat has been in the beamed nurses and breeding in the long time, its germ plasm was more limited. The breakthrough of wheat much more depended on exogenous genes, the researches that transferring those genes into wheat could effect on its quality or not has not almost studies, it was yet all-important to study.In order to probe the influence of transferring alien genes into common wheat on its processing quality, the progenies from a wide cross between one wheat (Triticum aestivum L) cultivar Mianyangll (MYll) having high yield character and rye (Secale cereal. L) in bred lines R3,R12,Bailli rye were studied in the present paper. Agronomy characters, cytology were researched, gliadin and HMW-glutenin subunits compositions were analysed, another SDS-sedimentation value was determined, so as to supply the theory ground in genetic improvement of wheat by wild cross and offer the references for breeding high quality wheat. The results are as follows:1.82 advance lines having steady agronomy character and cytogenetical stability were identified, in which 19 lines exhibited agronomy variations including plant height, spike shape, blossom stage and athera length. Another 46 lines had a pair of translocation chromosome 1BL/1RS through C-banding technique such as 859-4,1116 series, 1208 series and so on,.2. The gliadin patterns of those progenies were investigated. In comparison with their parent wheat MYll, 19 lines were the same pattern with MYll which were named wheat MY11 type short for MT. In addition, the gliadin composition in co area of 46 lines have the peculiarly strips of wheat -rye translocation 1BL/1RS such Gli-lBl which were named translocation type short for TT. Other 17 lines both different from wheat MYll and translocation 1BL/1RS were found variation patterns in " area. The result was possibly shown combination or mutation, those lines were named CMT.46 lines with a pair of T1BL/1RS chromosome were identified and judged to T1BL/1RS translocation lines by C-banding and A-PAGE.3. The high-molecular-weight-glutenin-subunit (HMW-GS) composition of those progenies were investigated by seed storage protein electrophoresis. The HMW-glutenin subunits of MT lines had no variation on the whole, yet on the contrary the subunits of the translocation 1BL/1RS lines almost changed in which the novel ones mainly display in the loci of Glu-Bl and Glu-Al. those results revealed translocation 1B/1R could effected HMW-Gs of progenies. In addition, the CMT lines was also exhibited variation which fasten on subunits encoded by Glu-Al and Glu-Dl.SDS-PAGE revealed that the genetic variation on HMW-GS were found and the variation subunits include N(a), 7+9(c), 20(e), 2+12(a) encoded by the Glu-Al,Glu-Bl,Glu-Dl. The glutenin composition of wheat MYll(l,7+8,5+10) were the most types in their three corresponding site in all progenies. However the novel subunits occupied different rate in different site. The variation degree ofthree site was Glu-Bl>Glu-Al>Glu-Dl.4.The SDS-sedimentation value (SDSS) of those progenies seed exhibited generally much higher than their parent MY11, the distance of mean value between T1BL/1RS and MT was not signifficent defference, those results revealed that the processing quality of progenies were better than wheat MY11, and IRS did not effected SDSS.5. The effect of HMW-GS were also analysed and found that SDSS of subunit 7+9,5+10 was not higher than other subunits which reason was discussed.6. The variation degree, stability, variation type were different from each other in progenies group of three wild crosses between one wheat cultivar MY11 and three rye inbred lines, the result was possibly shown that three rye lines had genetic diversity. In the progenies from wild cross between R3 and MY11, variation degree was higher and there were more variaties.7. The progenies from the distant hybrid between MY 11 and r... |
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