Studies On Regeneration And Gene Transformation Of Passiflora Quadrangularis L.

The keratinizative shell of the seed was denudated, then degermed by javel water(NaC10). The germfree seedlings were obtained by germfree germination in MS medium. Adventitious buds were induced from the cotyledons and hypocotyl inoculated in MS medium with BA and IAA of disparate concentration. The MS1 (MS+BA2.0mg/L+IAA0.1mg/L) is confirmed to be the most suitable for inducing adventitious buds. The adventitious buds were inoculated in MS medium with NAA and D3A of disparate concentration to induce adventitious roots. The MSa (MS+NAA 0.5mg/L+IBA0.2mg/L) was confirmed to be best for inducing adventitious root. The selective pressure was determinated by inoculating the cotyledons and hypocityls in MS1, and adventitious bud in MSi.A plant expression vector pBICr, earring in versed-connected CP gene amplified from pBIC by PCR with a pair of primers without restriction nuclease site, was constructed by inserting it into pBI121 plasmid, and was introduced into Agro-bacterium tumefaciens strain LBA4404. It was tranformed into P.quadrangularis L. via the Agrobacterium tumefaciens-msdiated method, and 9 kanamycin-resistant plantlets were obtained. Spot blotting confirm that the CMV-CP gene has been integrated into the genome of some of the P. quadrangularis L. plantlets. The establishing of regeneration system of P.quadrangularis L. will perfect the transgenic system of passiflora, and accelerate the development of passiflora trade.