| In order to find out regularity of antigenic vareation, a cloned population of Trypanosoma evansi stock YnTatl was infected sequentially to 5 New Zealand rabbits during 150 days. Competing the ablity of anti-trypanosoma evansi between 3 different antibody bank derived from rabbits, setting up by different means.At first, study on antigenic variation in trypanosoma evansi from a clonal stock in rabbit was carried out. A rabbit was infected with a cloned YnTatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non-cloned trypanosome population. Repeated above all, thus infected 5 rabbits sequentially. Twenty different VATs(variant antigen type) were monitored and characterized from those fifty mono-clonal populations by indirect immunofluorescence test(IFT) and avidin biotin enzyme immunoassay(ABC-EIA). They were designated as YnTat 1.1. YnTat 1.2 BeTat 1.3......BeTat 1.19. BeTat 1.20.Purified YnTatl trypanosomes were broken by N-tosyl-lysine chloromethyl ketone (TLCK) and centrifugated by high g to form soluble proteins. The soluble proteins were chromatographed on a DE-52 column. The material in the first peak constituted the purified VSG. The molecular weight of the purified VSG was 41.94KD by detection of SDS-PAGE.Three antibody banks were set up depending on 20 VATS. Some SD rats infected 100 trypanosomes were survived in Yuanhun and Activing-trypanosome antibody bank treated. VSG-antibody bank was similar to control. |
