Isolating And Cloning Of The Genes Conferring Plant Height In Common Wheat

The height of the wheat is one of the important characters. The variety with the dwarfing gene increase grain yield by the expense of straw biomass, and are more resistant to damage by wind and rain. Now, the transgenic plant is more and more. Isolating and cloning the dwarfing gene by conserved sequence and PCR cloning can be used to transfer to the common wheat and get suitable height of wheat.According to the reducing-height genes's conservative sequences which were insensive to GA and have been isolated and cloned from Maize, Arabidopsis and Wheat, this experiment designed six primers. With different primer pairs, this experiment amplified West-South NILs, Jing411 NILs and Jing 411 CK genomic DNA. Jing 411 NIL backcrossed with the common Jing 411 wheat to select new combination. The results were as follows:Acquired Jing 411 NILs, which include four members.The plant height genes in West-South 02 , West-South 05 , Rht10 gene, high straw gene and Jing 411 CK were got. Amplified product were sequenced, the result shows that comparing to wild high straw gene, West-South 02 has two bases mutation, West-South 05 has 12 bases mutation in different spots and 3 sequential bases lost, Rht10 gene has 4 bases mutation, high straw gene and Jing 411 CK has no mutation. They are 99.8%, 99.1%, 99.7%, 100%, 100% homogeneous to wild high straw gene respectively.Comparing to wide high straw gene amino-acid sequences, West-South 02 has one amino-acid mutation at the sixth functional region; West-South 05 has three amino-acids mutation and one amino-acid lost, and mutation and lost site are all in sixth and seventh functional region. RhtlO gene has three amino-acids mutation and the mutation site are in first, second and sixth region respectively.