| The different diameter colloidal gold particles were synthesized by reducing hydrogen tetrachloroaurate with sodium citrate using the microwave oven which was a heating up installation.At the same time, we gained the referenced data which prepared colloidal gold particles form 100 to 500ml. The 20nm colloidal gold particles were selected.according to the different diameter colloidal gold particles showing different color(easy to judge). The NDV was purified on the zonal ultracentrifuging at 4 ,then use the method of colloidal gold immunoelectronmicroscopy to detect NDV. The result showed that the NDV had been purified. Using the NDV immunized rabbit and chicken, the received serum was purified by caprilic acid/ammonium sulfamate precipitation. The purified antibodies were analyzed by the conventional PAGE and agar diffusion test.And the result showed that the IgG in high purity and good activities.The gold sol was adjusted to pH 9.0 then at 4癈, the labeled rabbit anti-NDV IgG was purify on zonal ultracentrifuging. We diluted the labeled rabbit anti-NDV IgG in PBS(containing 1% BSA) at the rate of 1: 2. The labeled antibodies was immobilized to nitrocellulose membrane(NC). The two kind of antibody concentration was optimized by the square matrix test. We selected the HF135 to prepare the test strip.Meanwhile, a optimized and stable formula was obtained. The test strip was placed in 4"C desiccator.after 8 month its effect of detection and releasing still hold the line. 157 clinical samples were detected by the strip comparing with HA, ELISA, The test strip show is easy to be justified;time-saving,sensitive,and suitable for at grassroot level and epidemiology investigate.These key techniques and the date will help us research and develop a series of GICA strip to detect the domestic animals and poultry.
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