Study On Transformation Of P.alba Var.Pyrarridalis And P.alba Var.Payrarridalis×P.alba L With A Gene Encoding For The Betaine A Aldehyde Dehydrogenase (BADH)

P.alba Var.pyrarridalis and P.alba Var.payrarridalisxP.alba L are important trees which are abroad plant in xinjiang of China.and are important for ecological environment and economy of the northwest.A gene encoding for the betaine aldehyde dehydrogenase(BADH) was transform to P.alba Var.pyrarridalis and P.alba Var.payrarridalisxP.alba L in this study. 17 km- resistant plants were obtained for P.alba Var.pyrarridalis and 21 km-resistant plants were obtained for P.alba Var.payrarridalisxP.alba L. The differentiation culture of P.alba Var.payrarridalisxP.alba L was optimized. The result showed that 1/2MS BA0.25mg/L+TDZ0.002 and 0.004mg/L+IAA0.5mg/L could meet the requirement of transformation. The frequency of differention of leaves disc reached 90.3% and 78.1%. Simultaneously, rooting culture was optimized. Using 1/2MS, IBA0.2mg/L ,carrageenan, the ratio of rooting could reach 100%.The effect of km on differentiation of leaves discs of P.alba Var.payrarridalisxP.alba L was studied. The result showed when the concentration of km reached 15mg/L, the leaves discs could not differentiate. So km 15mg/L or 20mg/L were adopted to screen leaves discs. For leafstalks, when the concentration of km reached to 25mg/L, they could not regenerate bud. So km 25mg/L was adopted to screen leafstalks. For the sake of increasing the pressure of screen, the effect of km on rooting was studied. The result showed that when the concentration of km reached to 10mg/L, the plant could not generate root. Thus km 10mg/L was adopted to screen rooting.Groping the system of transformation, it was indicated that the addition of As 100mg/L as co-culturing leaves discs could increase the transformative frenquency; and when infecting leaves discs for 15min-20min, the transformative frequency was the best; and when properly increasing the concentration of cytokinin TDZ, the transformative frequency could be increased. Simultaneously, when using YEB culture to infect leaves discs, km resistant plant could not be obtained. Through screening 3341 leaves discs, 16 km resistence bud was obtained. The transformative frequency was about 0.5%.PCR testing km-resistant plant of P.alba Var.pyrarridalis, the result showed that 2 plants was positive among 17 km resistence plants. Using primer 1 and 3, positive band could be obtained in xy11 and xy12. And using primer 2 and 3, all thekm-resistant plant including ck- could amplify special band. The band was between 300-400bp. Using primer 1 and 2 of the gene of CpTI, testing transformative plant of CpTI, a positive plant was obtained.