Construction Of A Molecular Genetic Map For Watermelon Derived From Recombinant Inbred Lines (RIL)

A molecular genetic map was constructed for watermelon based on 87 RAPD(random amplified polymorphic DNAs) markers, 13 ISSR(inter simple sequence repeat) markers, and 4 SCAR(sequence characterized amplified region) markers using a RIL population of 117 F8 progenies derived from the cross 97103(which is a cultivar with higher total soluble solids concentration, thin rind, susceptible to Fusarium wilt disease) X PI296341 (which is a wild germplasm with lower total soluble solids concentration, thick rind, resistant to Fusarium wilt disease). The map consists of 15 linkage groups. Among them are a large groups linkage group that contains 31 markers covering a mapping distance of 277. 5 cM, six large groups each with 4-12 markers covering a mapping distance of 51. 7 to 172. 2c M and eight small groups each consisting of 2-5 markers covering a mapping distance of 7.9-46.4 cM. The entire map covers a total distance of 1027. 5cM with an average distance of 11.54 cM between two markers. This map is useful for the further development of matured molecular genetic map, analysis of important agricultural characterized quantitative trait loci (QTL) and map-based positional cloning for disease resistance gene.