Phylogeny Of Genus Of Bean (Phaseolus Vulgaris.L.) In Heilongjiang Province By RAPD

The experiments were on the basis of the observation in the fields. 15 cultivars were selected out from 27 special cultivars . judging by biological characters and performance, which includes 12 for vine. and 3 for dwarf. The total amount of 27 cuhivars was systematically studied and discussed for two aspects: biological characters and RAPD molecular markers. The results showed:1) The experiments on fresh bean in the fields showed that special local beans of Heilongjiang Province are good varieties of common bean, and they are the results of seed-selection of decades of years.. The natural resources of them in Heilongjiang province were abundant, but no systematically nomenclature existed. On the respect of biological characters, some color of blosom can distinguish them from common beans during the different periods of growth.2) In the section of molecular biological experiments, the modified SDS method which was used to extract DNA got good results. Using RNAse could eliminate the disturbance of RNA. and got the qualified DNA with a long term of validity. The optimized RAPD systems were: total reaction volume 251. including 10 times amplification buffer solution 2.51. Mg2+ 2.5mmol/1. dNTP 300 mol/l, 0.1mmol/1, genome DNA 60ng. Taq enzymel.5U. Optimized program: 94 C 10 min; 94 C 1 min ,37 C 1 min, 72 C1mm . forty cycles; 72 C 7min one cycle; 4 C keeping. 27 cultivars were detected by RAPD through 14 selected arbitrary primers. A total of 117 loci were amplified among which 104 loci were polymorphic with a mean of bands per primer. The ratio of polymorphism is 89.9%. The genetic similar degree is 6.90 as threshold. Cluster (UPGMA) and principal coordinate analyses identified beans of Heilongjiang province as a distinct group with the rest. The result is in harmony with biological feature.Genetic similar degree is 6.5 as threshold,cluster (UPGMA) and coordinate analysis identified beans as 7groups which is similar to geographical areas.3) The primer SBSF-5 can distinguished 25 cultivars by RAPD method.Candidate:SunXiaodan Mayor. Vegetable breedingSupervisor: Prof.QinZhiwei...