The Preliminary Study On Detection Of GMO In Agriculture

The first Genetically Modified Crops (GMC), which was born in the 1983 maybe have brought up potential risk when it gave human much favor. As foods produced from Genetically Modified organisms (GMOs) or food products containing GMOs are appearing more and more frequently on the market, the problem of biosafety is being extensively debated and resulted kinds of conflict on politics , trade and even religion. As a result, many countries promulgated laws and regulation on GMO biosafety. Our government required the agricultural products marketed in china such as soybean, maize , their products and so on ,if it produced from GMOs or contained GMOs, must be labeled before flowed into market A feasible detection method should be built to meet the needs from foods labelling, producer and consumers.In this study, a PCR detection method on CaMV 35S promoter and nos terminator which are extensively used in GMC as regulatory elements. The PCR detection result showed: the practical detection limit was 0.5%(w/w), which meant this method could detect the GM soybean when its weight proportion was equal or exceeded 0.5%. Another detection method, dot blotting, was built also based on 35S promoter and nos terminator. The two genes were labeled by digoxin to done as probe used in dot blotting. The blotting detection showed: the PCR detection on 35S and nos is correct, while when the GMS content decrease the detection signal weakened. So this method was not fit to be applied in market detection.