The Establishment Of Determining Method Of Glutamine In Milk And The Study Of Dynamic Changes In Sows Milk

There are three series in this experiment, including the establishment a new high performance liquid chromatography method for determining the concentration of glutamine in livestock milk, and the determination of the dynamic changes on glutamine concentration of sows colostrums and milk, and the study of growing performance effect of glutamine on early weaning diglets.In series I, We have established a new performance liquid chromatography method that is accurate, simple and quick for determining the concentration of glutamine in milk. Gin was derived with phenylisothiocyanate (PETC) and diluted with 50mmol/L sodium acetate (pH6.50) containing 23% acetonitrile on reverse phase CIS column. The derivative was detected at 254nm. The result was showed as follows: calibration on curves for Gin was linear from 25 to 500μmol/L; linear formula is A=31412.8C±111998.39; r=0.9987 and CV was between 3.64%; The mean recovery was 104.6% and 99.9%. the normal concentration was 46.8 ±21.5/玭ol/L in health adults cow (the third months after parturition), was 352.4±36.5μmol/L in goat (the 25th days after parturition), was 29.22±15.1μmol/L in pig (the first day after parturition) .We can draw a conclusion from the experiment that the method is simple, quick and efficient and that the method is suit to determining the concentration of glutamine in animal milk.In series II, We used ten dominant swine species-Landrace ×Yorkshire as aresearch model. We collected Milk once two days from the 1st day to the 35th day after parturition from 10 sows similarly, about each 10:00 AM. The concentration of Gin was determined as soon as possible after collecting the samples of milk. The method of determining is the same as the series I . The result was showed as follows: The concentration of free Gin was 29.22μmol/L in the first day of post-parturition and increased gradually with the time passing, and reached the summit in the 27th day of post-parturition that was 593.74μmol/L. There was a fluctuation from 13th post-parturition to 21rd post-parturition, and the lowest of values expressed in the 17th post-parturition. We can draw a conclusion: the result conform to that of forecast, because Gin can accelerate the growth and mature of piglet intestinal epithelial cell, and maintain the villus height, and decrease the lamina depth. To improve the rate of parturition, piglet was always weaned early (the 21st). The concentration of glutamine increasing gradually between the 21st and the 27th, if piglet was weaned in the 21st, the outer-glutamine of piglet was broken off. Piglet will have a diarrhea because of the lack of glutamine.In series III, 100 piglets at the 21st of age from 12 normal 3-4years DurocX Landrace X Yorkshire parturition sows were randomly assigned to one of four dietary treatments(0,0.5,1.0,1.5% glutamine supplementation). The growing performance was determined. The Average daily growth of group 1.0% glutamine was the highest, and the diarrhea rate of which was the lowest, comparing with the control group. We can draw a conclusion: Dietary glutamine supplementation improved growth performance, reduced the diarrhea and the death rate of piglets, reduced the fall of plasma glutamine level after weaned. The effect of supplement glutamine 1.0% group was the best.