| This experiment of distant hybridization has been done in fruit breeding base of Shandong Agriculture University in Hengling ,fruit-biology laboroty of Shandong Agriculture University, Nanxu orchard in Shengzhuang and Sijiazhuang orchard. Eleven varieties of stone fruits ( peach , apricot ,plum ,cherry and apricot-plum ) were used in it . More than seventy cross-combinations were disigned between different stone fruits , and their cross-compatibility was simply explored. In addition , in order to improve cross-compatibility of distant hybridization , some treatments were disigned in some cross-combinations, such as electric field treatments of different intensity, He-Ne laser treatments of different time and γ-ray radiation treatments of different dosage, hormone treatments and pollination with compound pollen . Moreover, budding energy of paternal pollen were tested . Finally, seedlings of hybrids were identified by RAPD. The result shows : 1. The result of the experiment of distant hybridization between peach , apricot , plum and apricot-plum indicates : ①The effect of different stone fruits as female parents on cross-compatibility of distant hybridization is remarkable . Among the cross-combinations with four species of stone fruits as female parents , the average setting percentage respectively is : peach , 35.8% , the highest ; apricot , 16.3% , the second ; apricot-plum , 6.3% , the third ; plum , 1.9% , the lowest .②The setting percentage of apricot-plum × apricot is 12.0% , obviously higher than apricot-plum × plum ,which is 2.7% . So deos apricot × apricot-plum and plum × apricot-plum . This result indicates this variety of apricot-plum used in the experiment has closer relationship with apricot than with plum . Furthermroe ,it proves the relationship between different fruits can also be expored by cross-compatibility of distant hybridization . 2. The effect of different varieties ( genetypes ) of apricot as female parents on cross-compatibility of distant hybridization is remarkable . Among the cross-combinations with three varieties of apricot as female parents , theaverage setting percentage of the cross-combinations with apricot (hongfeng) as female parents is highest , 37.1% ; apricot(xinshiji) , second , 10.7%; apricot(xinshiji) , lowest , 2.3% . However ,The effect of different varieties of apricot as male parents on cross-compatibility of distant hybridization is not remarkable .3. The result of the experiment of distant hybridization with pollen treated by laser , electric field andγ-ray indicates : ①The appropriate electric field (434.78 Kv/m) and He-Ne laser (8min) treating paternal pollen can effectively improve cross-compatibility of cherry ( Hongfeng ) ×apricot ( Badan ). The effect with the optimum He-Ne laser treating paternal pollen is best , the electric field treatment next,and the γ-ray radiation treatment not remarkable . ②The effect of the compound treatment with 500G γ-ray and 5min He-Ne laser on improving cross-compatibility of distant hybridization is remarkablely better than that of either of them alone . For example ,the setting percentage of cherry ( Hongfeng ) × peach ( Shuguangyoutao ) pollinated with compoundly treated pollen is 24.4％,obviously higher than that of either of alone treatment of 500G γ-ray (4.8%) and 5min He-Ne laser (3.4%) ,or comparision (5.0%) . 4. The result of the experiment of distant hybridization with hotmone treating maternal ovary or stigma and pollinated with compound pollen indicate that both hormone (GA and β-NAA) treatments and compound pollen pollination can,t improve cross-compatibility of apricot ( Badan shuixing) × apricot-plum , apricot ( Badan ) × plum (dashizaosheng) , apricot ( Badan ) × peach (shuguangyoutao). The setting percentage of all the treated cross-combinations is zero.5. The result of the experiment of distant hybridization pollinated in different blooming stage indicates that setting percentage is different in different pollination stage and it is obviously higher in balloon...
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