| As a kind of precious traditional Chinese medicine, The ginseng not only occupy the important status in the Traditional Chinese medicine field of our country, also quite paid attention to in country's medical science, such as Japan, Korea, the former Soviet Union, etc. In recent years, the pharmacology research about the ginseng has already entered the stage for rapidly development. A large number of research proves that the glucoside of ginsengs is the main active composition of the ginseng. Prove that the soap glucoside of ginsengs is a kind of very good immunity strengthens the pharmaceutical through animal's experiment, It is strengthening the systematic function of single-nuclear macrophage cell, promotes the formulation antibody and lymphocyte transformed, Promote some lymph secretion of factor, play a regulating role in natural killer cell-interferon-IL-2 regulate network, and protect the organization cell attacked by the virus. Though someone reports that roots ethanol extract of ginseng have function of urging to grow on the chicken, there is not the one that has been used directly in animal medicine. The stem leaf glucoside of American ginseng is the total glucoside drawn from the stem leaf of American ginseng, And verify that the content of glucoside of the total soap is obviously higher than roots in the leaf of stems. As the big culture province of ginseng, there are a lot of ginseng leaves as the offal abandon in the field but not to fully utilized every year in Jilin Province. It is abundant to develop, utilize the leaf of ginsengs to have resources, It have much merits such as safe and convenient, without residue, and contain many kinds of active composition. Feed the animal directly as the additive, It can prevented disease and urge to grow role. With the development of the green ecological animal husbandry and constant improvement of people's living standard after the accession to the WTO, The application of leaf of ginseng has a bright future in the aquaculture. The purpose of tests is to study the regulating action to chicken's immune function of glucoside of soap of leaf of ginsengs, Offer basis on which develop the application in the aquaculture of leaf of ginseng.The test adopts single factor variance and analyses, All chicken divides into 5groups, 1 groups is the contrast group, The others are test groups, every group set up three repetitions, 20 chicken in each repetitions (2 group feed the fodder including 5% of the ginseng leaf to the 35th age since the 1st age; 3 groups hypodermic injected of 5% of the ginseng glucoside physiological saline once from 14 to the 20th age, Dosage is 20mg/kg; 4 groups hypodermic injected of 5% of the ginseng glucoside physiological saline once from 28 to the 35th age, Dosage is 20mg/kg; 5 groups hypodermic injected ND oil containing soap glucoside of the ginseng in the 21st age. Routine hypodermic injected immunity ND oil of every other groups).Collect of 6 chickens at random in 2 groups, 4 groups, Each chicken were intravenous injection E.coli lml(include 4xl06/ml). Makes the plateform to examines the counts in blood and after Ih, 2h, 3h pouring into E.coli.Weight 10 develop normal chickens on an empty stomach at random from 1~3 groups, death through arterial carotis bloodletting, separated and determined chest gland, bursa and spleen weight and index.At the time of 1d, 7d, 14d, 21d, 28d, 35d 42d after second immunity, Collect 10 chickens at random from 1 groups, 2groups, 3groups, Sgroups, collect blood 0.5 ml in wing vein, separate the serum, Determine the HI antibody price in serum with p-micro-methods.The result of study shows: Compared with contrast group, the leaf glucoside of the ginseng can promote chickens to resist the production of NDV antibody; The leaf glucoside of the ginseng has facilitation to the development of the pigeon breast gland, have inhibition on the development of chicken's spleen, Different influence of bursa because of different way when giving the medicine; Ginseng leaf glucoside can formed ability of nucl... |
